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dc.contributor.authorSchneider, Olha
dc.contributor.authorSimic, Nebojsa
dc.contributor.authorAachmann, Finn Lillelund
dc.contributor.authorRückert, Christian
dc.contributor.authorKristiansen, Kåre Andre
dc.contributor.authorKalinowski, Jörn
dc.contributor.authorJiang, Yi
dc.contributor.authorWang, Lisong
dc.contributor.authorJiang, Cheng-Lin
dc.contributor.authorLale, Rahmi
dc.contributor.authorZotchev, Sergey B
dc.date.accessioned2019-09-13T06:37:05Z
dc.date.available2019-09-13T06:37:05Z
dc.date.created2019-01-09T09:47:52Z
dc.date.issued2018
dc.identifier.citationFrontiers in Microbiology. 2018, 9 (3139), .nb_NO
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/11250/2616670
dc.description.abstractStreptomyces bacteria are recognized as an important source for antibiotics with broad applications in human medicine and animal health. Here, we report the isolation of a new lichen-associating Streptomyces sp. YIM 130001 from the tropical rainforest in Xishuangbanna (Yunnan, China), which displayed antibacterial activity against Bacillus subtilis. The draft genome sequence of this isolate strain revealed 18 putative biosynthetic gene clusters (BGCs) for secondary metabolites, which is an unusually low number compared to a typical streptomycete. Inactivation of a lantibiotic dehydrogenase-encoding gene from the BGC presumed to govern biosynthesis of a thiopeptide resulted in the loss of bioactivity. Using comparative HPLC analysis, two peaks in the chromatogram were identified in the extract from the wild-type strain, which were missing in the extract from the mutant. The compounds corresponding to the identified peaks were purified, and structure of one compound was elucidated using NMR. The compound, designated geninthiocin B, showed high similarity to several 35-membered macrocyclic thiopeptides geninthiocin, Val-geninthiocin and berninamycin A. Bioinformatics analysis of the geninthiocin B BGC revealed its close homology to that of berninamycins.nb_NO
dc.language.isoengnb_NO
dc.publisherFrontiers Medianb_NO
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleGenome Mining of Streptomyces sp. YIM 130001 Isolated From Lichen Affords New Thiopeptide Antibioticnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.source.pagenumber12nb_NO
dc.source.volume9nb_NO
dc.source.journalFrontiers in Microbiologynb_NO
dc.source.issue3139nb_NO
dc.identifier.doi10.3389/fmicb.2018.03139
dc.identifier.cristin1652937
dc.description.localcode© 2018 Schneider, Simic, Aachmann, Rückert, Kristiansen, Kalinowski, Jiang, Wang, Jiang, Lale and Zotchev. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.nb_NO
cristin.unitcode194,66,15,0
cristin.unitcode194,66,25,0
cristin.unitnameInstitutt for bioteknologi og matvitenskap
cristin.unitnameInstitutt for kjemi
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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