dc.contributor.author | Schneider, Olha | |
dc.contributor.author | Simic, Nebojsa | |
dc.contributor.author | Aachmann, Finn Lillelund | |
dc.contributor.author | Rückert, Christian | |
dc.contributor.author | Kristiansen, Kåre Andre | |
dc.contributor.author | Kalinowski, Jörn | |
dc.contributor.author | Jiang, Yi | |
dc.contributor.author | Wang, Lisong | |
dc.contributor.author | Jiang, Cheng-Lin | |
dc.contributor.author | Lale, Rahmi | |
dc.contributor.author | Zotchev, Sergey B | |
dc.date.accessioned | 2019-09-13T06:37:05Z | |
dc.date.available | 2019-09-13T06:37:05Z | |
dc.date.created | 2019-01-09T09:47:52Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Frontiers in Microbiology. 2018, 9 (3139), . | nb_NO |
dc.identifier.issn | 1664-302X | |
dc.identifier.uri | http://hdl.handle.net/11250/2616670 | |
dc.description.abstract | Streptomyces bacteria are recognized as an important source for antibiotics with broad applications in human medicine and animal health. Here, we report the isolation of a new lichen-associating Streptomyces sp. YIM 130001 from the tropical rainforest in Xishuangbanna (Yunnan, China), which displayed antibacterial activity against Bacillus subtilis. The draft genome sequence of this isolate strain revealed 18 putative biosynthetic gene clusters (BGCs) for secondary metabolites, which is an unusually low number compared to a typical streptomycete. Inactivation of a lantibiotic dehydrogenase-encoding gene from the BGC presumed to govern biosynthesis of a thiopeptide resulted in the loss of bioactivity. Using comparative HPLC analysis, two peaks in the chromatogram were identified in the extract from the wild-type strain, which were missing in the extract from the mutant. The compounds corresponding to the identified peaks were purified, and structure of one compound was elucidated using NMR. The compound, designated geninthiocin B, showed high similarity to several 35-membered macrocyclic thiopeptides geninthiocin, Val-geninthiocin and berninamycin A. Bioinformatics analysis of the geninthiocin B BGC revealed its close homology to that of berninamycins. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Frontiers Media | nb_NO |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Genome Mining of Streptomyces sp. YIM 130001 Isolated From Lichen Affords New Thiopeptide Antibiotic | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | publishedVersion | nb_NO |
dc.source.pagenumber | 12 | nb_NO |
dc.source.volume | 9 | nb_NO |
dc.source.journal | Frontiers in Microbiology | nb_NO |
dc.source.issue | 3139 | nb_NO |
dc.identifier.doi | 10.3389/fmicb.2018.03139 | |
dc.identifier.cristin | 1652937 | |
dc.description.localcode | © 2018 Schneider, Simic, Aachmann, Rückert, Kristiansen, Kalinowski, Jiang, Wang, Jiang, Lale and Zotchev. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | nb_NO |
cristin.unitcode | 194,66,15,0 | |
cristin.unitcode | 194,66,25,0 | |
cristin.unitname | Institutt for bioteknologi og matvitenskap | |
cristin.unitname | Institutt for kjemi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |