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dc.contributor.advisorSikorski, Pawel Tadeusz
dc.contributor.advisorKöllensperger, Peter
dc.contributor.authorEide, Ane Tefre
dc.date.accessioned2019-09-11T09:57:14Z
dc.date.created2016-06-15
dc.date.issued2016
dc.identifierntnudaim:14987
dc.identifier.urihttp://hdl.handle.net/11250/2615563
dc.description.abstractIn the search of more sensitive and portable biological sensors for medical diagnostics, a novel electrochemical biosensor is being developed. The sensor is based on a chip with an array of ring-recessed disk electrodes, where nanometre-sized holes are etched through a sandwich of thin metal layers with an insulating layer of silicon nitride in between. This double electrode geometry is foreseen to have far reaching applications in several different areas, and in particular detection of sub micromolar analytes. The detection scheme is based on biofunctionalisation of the sidewalls of the etched holes, where recognition elements can be conjugated to the silicon nitride. The first part of this study has thus been focused on development and characerisation of a protocol for functionalisation of silicon nitride by silanisation. The silane layer has been characterised by \gls{ca}, \gls{afm}, \gls{SE} and \gls{xps}. Biotin has then been attached to the end groups on the silane via a \gls{peg} spacer. Biotin is a small molecule that can bind with high affinity to streptavidin, which can function as a cross-linker to other biotinylatated entities, e.g. antibodies or enzymes. A streptavidin conjugated fluorophore has been used as proof-of-concept to confirm cross-linking to biotin. A procedure was found which resulted in thin, uniform silane layers suitable for further conjugation chemistry, and biotin was confirmed successfully cross-linked to the surface. This protocol is a good starting point for different kinds of functionalisation of the actual device, as well as for other projects where it is desired to conjugate biological entities to silicon nitride surfaces. The second part of this work focused on electrochemical characterisation of prototype devices without functionalised sidewalls, to check the response of the microelectrode system run in what is know as generator-collector (GC) mode. This has been done by cyclic voltammetry measurements, where a potential has been linearly swept at the bottom electrodes, while keeping the potential at the top electrodes constant. The measurements served to reveal several important aspects to consider for future work in order to improve the device quality, and also gave promising indications on the possible use of the device in multiplexed applications. Work remains to combine the functionalisation procedure with an actual device, and the electrochemical characteristics need improvement. Suggestions have been made based on the obtained results, and if successful, the device could serve as a versatile platform for various sensing applications in the future.en
dc.languageeng
dc.publisherNTNU
dc.subjectNanoteknologi, Bionanoteknologien
dc.titleDevelopment and Characterisation of a Nanotechnology-Enhanced Electrochemial Biosensoren
dc.typeMaster thesisen
dc.source.pagenumber168
dc.contributor.departmentNorges teknisk-naturvitenskapelige universitet, Fakultet for naturvitenskap,Institutt for fysikknb_NO
dc.date.embargoenddate10000-01-01


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