| dc.description.abstract | Rheumatoid arthritis is a chronic, progressive, inflammatory autoimmune disease that can cause severe joint destruction and disability. There have been major strides in understanding how the inflammatory process leads to joint destruction, in spite of the information scarcity of the etiology of RA. Several cell processes contribute to joint destruction in RA, including synovial immune complex deposition, neutrophil infiltration, angiogenesis, and T-cell activation. T-cell activation triggered by an unknown antigen leads to combined inflammation and destruction.
Confocal microscopy gives the possibility of obtain high quality images, however, the effectively use the confocal images relies on the handling and processing of digital images for analysis. There is still the need of research regarding the quantification of the fluorescence intensity extracted out of the confocal microscope. Here, we attempted to establish a method to quantify from the images taken the nuclear translocation of the subunit p65 of Nuclear Factor kB. Some technical and biological challenges were present along the course of the experiments performed, hence we were able to show p65 translocation, but to a lesser extent to quantify any reduction in nuclear p65 staining | en |
