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dc.contributor.authorKusnierczyk, Anna
dc.contributor.authorTran, Thi Diem Hong
dc.contributor.authorWinge, Per
dc.contributor.authorJørstad, Tommy Stokmo
dc.contributor.authorReese, John C
dc.contributor.authorTroczynska, Joanna
dc.contributor.authorBones, Atle M.
dc.date.accessioned2019-08-27T07:34:34Z
dc.date.available2019-08-27T07:34:34Z
dc.date.created2011-02-15T18:51:29Z
dc.date.issued2011
dc.identifier.citationBMC Genomics. 2011, 12 .nb_NO
dc.identifier.issn1471-2164
dc.identifier.urihttp://hdl.handle.net/11250/2611081
dc.description.abstractBackground Phloem-feeding aphids deprive plants of assimilates, but mostly manage to avoid causing the mechanical tissue damage inflicted by chewing insects. Nevertheless, jasmonate signalling that is induced by infestation is important in mediating resistance to phloem feeders. Aphid attack induces the jasmonic acid signalling pathway, but very little is known about the specific impact jasmonates have on the expression of genes that respond to aphid attack. Results We have evaluated the function that jasmonates have in regulating Arabidopsis thaliana responses to cabbage aphid (Brevicoryne brassicae) by conducting a large-scale transcriptional analysis of two mutants: aos, which is defective in jasmonate production, and fou2, which constitutively induces jasmonic acid biosynthesis. This analysis enabled us to determine which genes' expression patterns depend on the jasmonic acid signalling pathway. We identified more than 200 genes whose expression in non-challenged plants depended on jasmonate levels and more than 800 genes that responded differently to infestation in aos and fou2 plants than in wt. Several aphid-induced changes were compromised in the aos mutant, particularly genes connected to regulation of transcription, defence responses and redox changes. Due to jasmonate-triggered pre-activation of fou2, its transcriptional profile in non-challenged plants mimicked the induction of defence responses in wt. Additional activation of fou2 upon aphid attack was therefore limited. Insect fitness experiments revealed that the physiological consequences of fou2 mutation contributed to more effective protection against B. brassicae. However, the observed resistance of the fou2 mutant was based on antibiotic rather than feeding deterrent properties of the mutant as indicated by an analysis of aphid feeding behaviour. Conclusions Analysis of transcriptional profiles of wt, aos and fou2 plants revealed that the expression of more than 200 genes is dependent on jasmonate status, regardless of external stimuli. Moreover, the aphid-induced response of more than 800 transcripts is regulated by jasmonate signalling. Thus, in plants lacking jasmonates many of the defence-related responses induced by infestation in wt plants are impaired. Constant up-regulation of jasmonate signalling as evident in the fou2 mutant causes reduction in aphid population growth, likely as a result of antibiotic properties of fou2 plants. However, aos mutation does not seem to affect aphid performance when the density of B. brassicae populations on plants is low and aphids are free to move around.nb_NO
dc.language.isoengnb_NO
dc.publisherBioMed Centralnb_NO
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleTesting the importance of jasmonate signalling in induction of plant defences upon cabbage aphid (Brevicoryne brassicae) attacknb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.source.pagenumber16nb_NO
dc.source.volume12nb_NO
dc.source.journalBMC Genomicsnb_NO
dc.identifier.doi10.1186/1471-2164-12-423
dc.identifier.cristin538803
dc.description.localcodeOpen Access CC-BY 4.0nb_NO
cristin.unitcode194,66,10,0
cristin.unitnameInstitutt for biologi
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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