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dc.contributor.authorOlaisen, Camilla
dc.contributor.authorKvitvang, Hans Fredrik Nyvold
dc.contributor.authorLee, Sungmin
dc.contributor.authorAlmaas, Eivind
dc.contributor.authorBruheim, Per
dc.contributor.authorDrabløs, Finn
dc.contributor.authorOtterlei, Marit
dc.identifier.citationFEBS Open Bio. 2018, 8 (7), 1135-1145.nb_NO
dc.description.abstractProliferating cell nuclear antigen (PCNA), a member of the highly conserved DNA sliding clamp family, is an essential protein for cellular processes including DNA replication and repair. A large number of proteins from higher eukaryotes contain one of two PCNA‐interacting motifs: PCNA‐interacting protein box (PIP box) and AlkB homologue 2 PCNA‐interacting motif (APIM). APIM has been shown to be especially important during cellular stress. PIP box is known to be functionally conserved in yeast, and here, we show that this is also the case for APIM. Several of the 84 APIM‐containing yeast proteins are associated with cellular signaling as hub proteins, which are able to interact with a large number of other proteins. Cellular signaling is highly conserved throughout evolution, and we recently suggested a novel role for PCNA as a scaffold protein in cellular signaling in human cells. A cell‐penetrating peptide containing the APIM sequence increases the sensitivity toward the chemotherapeutic agent cisplatin in both yeast and human cells, and both yeast and human cells become hypersensitive when the Hog1/p38 MAPK pathway is blocked. These results suggest that the interactions between APIM‐containing signaling proteins and PCNA during the DNA damage response is evolutionary conserved between yeast and mammals and that PCNA has a role in cellular signaling also in yeast.nb_NO
dc.publisherWiley Open Accessnb_NO
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleThe role of PCNA as a scaffold protein in cellular signaling is functionally conserved between yeast and humansnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.source.journalFEBS Open Bionb_NO
dc.description.localcode© 2018 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution Licensenb_NO
cristin.unitnameInstitutt for klinisk og molekylær medisin
cristin.unitnameInstitutt for bioteknologi og matvitenskap

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal