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dc.contributor.authorAilte, Ieva
dc.contributor.authorLingelem, Anne Berit Dyve
dc.contributor.authorKvalvaag, Audun Sverre Myrset
dc.contributor.authorKavaliauskiene, Simona
dc.contributor.authorBrech, Andreas
dc.contributor.authorKoster, Gerbrand
dc.contributor.authorDommersnes, Paul Gunnar
dc.contributor.authorBergan, Jonas
dc.contributor.authorSkotland, Tore
dc.contributor.authorSandvig, Kirsten
dc.date.accessioned2018-07-02T06:17:49Z
dc.date.available2018-07-02T06:17:49Z
dc.date.created2017-02-26T16:18:46Z
dc.date.issued2017
dc.identifier.citationTraffic : the International Journal of Intracellular Transport. 2017, 18 (3), 176-191.nb_NO
dc.identifier.issn1398-9219
dc.identifier.urihttp://hdl.handle.net/11250/2503848
dc.description.abstractIn this study, we have investigated how clathrin-dependent endocytosis is affected by exogenously added lysophospholipids (LPLs). Addition of LPLs with large head groups strongly inhibits transferrin (Tf ) endocytosis in various cell lines, while LPLs with small head groups do not. Electron and total internal reflection fluorescence microscopy (EM and TIRF) reveal that treatment with lysophosphatidylinositol (LPI) with the fatty acyl group C18:0 leads to reduced numbers of invaginated clathrin-coated pits (CCPs) at the plasma membrane, fewer endocytic events per membrane area and increased lifetime of CCPs. Also, endocytosis of Tf becomes dependent on actin upon LPI treatment. Thus, our results demonstrate that one can regulate the kinetics and properties of clathrin-dependent endocytosis by addition of LPLs in a head group size- and fatty acyl-dependent manner. Furthermore, studies performed with optical tweezers show that less force is required to pull membrane tubules outwards from the plasma membrane when LPI is added to the cells. The results are in agreement with the notion that insertion of LPLs with large head groups creates a positive membrane curvature which might have a negative impact on events that require plasma membrane invagination, while it may facilitate membrane bending toward the cell exterior.nb_NO
dc.language.isoengnb_NO
dc.publisherWileynb_NO
dc.titleExogenous lysophospholipids with large head groups perturb clathrin-mediated endocytosisnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.source.pagenumber176-191nb_NO
dc.source.volume18nb_NO
dc.source.journalTraffic : the International Journal of Intracellular Transportnb_NO
dc.source.issue3nb_NO
dc.identifier.doi10.1111/tra.12468
dc.identifier.cristin1454004
dc.relation.projectNorges forskningsråd: 179571nb_NO
dc.description.localcodeThis article will not be available due to copyright restrictions (c) 2017 by Wileynb_NO
cristin.unitcode194,63,35,0
cristin.unitnameInstitutt for elektroniske systemer
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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