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dc.contributor.authorGullaksen, Stein-Erik
dc.contributor.authorSkavland, Jørn
dc.contributor.authorGavasso, Sonia
dc.contributor.authorTosevski, Vinko
dc.contributor.authorWarzocha, Krzysztof
dc.contributor.authorDumrese, Claudia
dc.contributor.authorFerrant, Augustin
dc.contributor.authorGedde-Dahl, Thobias
dc.contributor.authorHellmann, Andrzej
dc.contributor.authorJanssen, Jeroen
dc.contributor.authorLabar, Boris
dc.contributor.authorLang, Alois
dc.contributor.authorMajeed, Mohammed Waleed
dc.contributor.authorMihaylov, Georgi
dc.contributor.authorStentoft, Jesper
dc.contributor.authorStenke, Leif
dc.contributor.authorThaler, Josef
dc.contributor.authorThielen, Noortje
dc.contributor.authorVerhoef, Gregor
dc.contributor.authorVoglova, Jaroslava
dc.contributor.authorOssenkoppele, Gert
dc.contributor.authorHochhaus, Andreas
dc.contributor.authorHjorth-Hansen, Henrik
dc.contributor.authorMustjoki, Satu
dc.contributor.authorSopper, Sieghart
dc.contributor.authorGiles, Francis
dc.contributor.authorPorkka, Kimmo
dc.contributor.authorWolf, Dominik
dc.contributor.authorGjertsen, Bjørn Tore
dc.identifier.citationHaematologica. 2017, 102 (8), 1361-1367.nb_NO
dc.description.abstractMonitoring of single cell signal transduction in leukemic cellular subsets has been proposed to provide deeper understanding of disease biology and prognosis, but has so far not been tested in a clinical trial of targeted therapy. We developed a complete mass cytometry analysis pipeline for characterization of intracellular signal transduction patterns in the major leukocyte subsets of chronic phase chronic myeloid leukemia. Changes in phosphorylated Bcr-Abl1 and the signaling pathways involved were readily identifiable in peripheral blood single cells already within three hours of the patient receiving oral nilotinib. The signal transduction profiles of healthy donors were clearly distinct from those of the patients at diagnosis. Furthermore, using principal component analysis, we could show that phosphorylated transcription factors STAT3 (Y705) and CREB (S133) within seven days reflected BCR-ABL1IS at three and six months. Analyses of peripheral blood cells longitudinally collected from patients in the ENEST1st clinical trial showed that single cell mass cytometry appears to be highly suitable for future investigations addressing tyrosine kinase inhibitor dosing and effect.nb_NO
dc.publisherFerrata Storti Foundationnb_NO
dc.rightsNavngivelse-Ikkekommersiell 4.0 Internasjonal*
dc.titleSingle cell immune profiling by mass cytometry of newly diagnosed chronic phase chronic myeloid leukemia treated with nilotinibnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.localcode©2017 Ferrata Storti Foundation Material published in Haematologica is covered by copyright. All rights are reserved to the Ferrata Storti Foundation. Use of published material is allowed under the following terms and conditions: Copies of published material are allowed for personal or internal use. Sharing published material for non-commercial purposes is subject to the following conditions:, sect. 3. Reproducing and sharing published material for commercial purposes is not allowed without permission in writing from the publisher.nb_NO
cristin.unitnameInstitutt for klinisk og molekylær medisin

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Navngivelse-Ikkekommersiell 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse-Ikkekommersiell 4.0 Internasjonal