Bioassay-guided isolation and identification of anti-inflammatory compounds in Sclerochloa Dura

Abstract

Sclerochloa dura was known to have anti-inflammatory properties and the aim of this thesis is to isolate and identify compounds that are present in fractions and has high inhibition level against arachidonic acid (AA) when tested in bioassay. The methanolic extracts of the plant were stored as fractions and contained several bioactive compounds. They were subjected to separation through various chromatographic methods and structures of major isolated compounds were elucidated using NMR and ESI-MS-TOF. The five compounds identified in the fractions are genistein-4 -O-glucoside, luteolin-4 -O-glucoside, chrysoeriol-7-O--glucoside, isovitexin and (2E)-3-(4-Hydroxy-3-methoxyphenyl)-N-propylacrylamide.