dc.contributor.author | Hegstad, Solfrid | |
dc.contributor.author | Havnen, Hilde | |
dc.contributor.author | Helland, Arne | |
dc.contributor.author | Falch, Berit Margrethe Hasle | |
dc.contributor.author | Spigset, Olav | |
dc.date.accessioned | 2018-01-30T09:38:05Z | |
dc.date.available | 2018-01-30T09:38:05Z | |
dc.date.created | 2017-10-31T15:32:06Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Journal of chromatography. B. 2017, 1061-1062 103-109. | nb_NO |
dc.identifier.issn | 1570-0232 | |
dc.identifier.uri | http://hdl.handle.net/11250/2480573 | |
dc.description.abstract | A method for enantiomeric separation and quantification of R/S-citalopram in serum was developed and validated using ultra-high performance supercritical fluid chromatography-tandem mass spectrometry (UHPSFC-MS/MS). Sample preparation prior to UHPSFC-MS/MS analysis consisted of protein precipitation with acidic acetonitrile and filtration through a phospholipid removal plate. The UHPSFC-MS/MS method used an UPC2 Trefoil CEL2 column with a mobile phase consisting of CO2 and methanol/acetonitrile (70:30, v/v) with 10 mM ammonium acetate. The injection volume was 1 μL and run time was 4 min. MS/MS detection was performed with positive electrospray ionization and two multiple reaction monitoring transitions (m/z 325.1 > 262.0 and m/z 325.1 > 109.0). The calibration range was 5–500 nM for each analyte. The between-assay relative standard deviations were in the range of 3.4–4.5%. Recovery was 81–91% and matrix effects ranged from 96 to 101% (corrected with internal standard). After development and initial testing, the method has been successfully implemented in routine use in our laboratory for both separation and quantification of R/S-citalopram in more than 250 serum samples for therapeutic drug monitoring. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Elsevier | nb_NO |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no | * |
dc.title | Enantiomeric separation and quantification of citalopram in serum by ultra-high performance supercritical fluid chromatography-tandem mass spectrometry | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | acceptedVersion | nb_NO |
dc.source.pagenumber | 103-109 | nb_NO |
dc.source.volume | 1061-1062 | nb_NO |
dc.source.journal | Journal of chromatography. B | nb_NO |
dc.identifier.doi | 10.1016/j.jchromb.2017.07.009 | |
dc.identifier.cristin | 1509454 | |
dc.description.localcode | © 2017. This is the authors’ accepted and refereed manuscript to the article. Locked until 9.7.2019 due to copyright restrictions. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | nb_NO |
cristin.unitcode | 194,65,15,0 | |
cristin.unitname | Institutt for klinisk og molekylær medisin | |
cristin.ispublished | true | |
cristin.fulltext | postprint | |
cristin.qualitycode | 1 | |