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dc.contributor.authorEsmaeili, Morteza
dc.contributor.authorBathen, Tone Frost
dc.contributor.authorEngebråten, Olav
dc.contributor.authorMælandsmo, Gunhild
dc.contributor.authorGribbestad, Ingrid S
dc.contributor.authorMoestue, Siver Andreas
dc.date.accessioned2017-11-24T11:57:50Z
dc.date.available2017-11-24T11:57:50Z
dc.date.created2013-07-30T12:54:08Z
dc.date.issued2014
dc.identifier.citationMagnetic Resonance in Medicine. 2014, 71 (6), 1973-1981.nb_NO
dc.identifier.issn0740-3194
dc.identifier.urihttp://hdl.handle.net/11250/2468010
dc.description.abstractPURPOSE: Phospholipid metabolites are of importance in cancer studies, and have been suggested as candidate metabolic biomarkers for response to targeted anticancer drugs. The purpose of this study was to develop a phosphorus (31P) high resolution magic angle spinning (HR-MAS) magnetic resonance spectroscopy (MRS) protocol for quantification of phosphorylated metabolites in intact cancer tissue. METHODS: 31P spectra were acquired on a 14.1 T spectrometer with a triplet 1H/13C/31P MAS probe. Quantification of metabolites was performed using the PULCON principle. Basal-like and luminal-like breast cancer xenografts were treated with the dual PI3K/mTOR inhibitor BEZ235, and the impact of treatment on the concentration of phosphocholine (PCho), glycerophosphocholine (GPC), phosphoethanolamine (PEtn) and glycerophosphoethanolamine (GPE) was evaluated. RESULTS: In basal-like xenografts, BEZ235 treatment induced a significant decrease in PEtn (-25.6%, P = 0.01) whilst PCho (16.5%, P = 0.02) and GPC (37.3%, P < 0.001) were significantly increased. The metabolic changes could partially be explained by increased levels of phospholipase A2 group 4A (PLA2G4A). CONCLUSION: 31P HR-MAS MRS is a useful method for quantitative assessment of metabolic responses to PI3K inhibition. Using the PULCON principle for quantification, the levels of PCho, GPC, PEtn and GPE could be evaluated with high precision and accuracy.nb_NO
dc.language.isoengnb_NO
dc.publisherWileynb_NO
dc.titleQuantitative 31P HR-MAS MR spectroscopy for detection of response to PI3K/mTOR inhibition in breast cancer xenograftsnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionacceptedVersionnb_NO
dc.source.pagenumber1973-1981nb_NO
dc.source.volume71nb_NO
dc.source.journalMagnetic Resonance in Medicinenb_NO
dc.source.issue6nb_NO
dc.identifier.doi10.1002/mrm.24869
dc.identifier.cristin1040877
dc.relation.projectNorges forskningsråd: 221879nb_NO
dc.description.localcode© 2013 Wiley Periodicals, Inc. This is the peer reviewed version of the article, which has been published in final form at dx.doi.org/10.1002/mrm.24869. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.nb_NO
cristin.unitcode194,65,25,0
cristin.unitnameInstitutt for sirkulasjon og bildediagnostikk
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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