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dc.contributor.authorOlsen, Oddrun Elise
dc.contributor.authorSkjærvik, Anette
dc.contributor.authorStørdal, Berit Fladvad
dc.contributor.authorSundan, Anders
dc.contributor.authorHolien, Toril
dc.date.accessioned2017-11-23T07:29:32Z
dc.date.available2017-11-23T07:29:32Z
dc.date.created2017-11-22T08:32:10Z
dc.date.issued2017
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11250/2467665
dc.description.abstractPurified recombinant proteins for use in biomedical research are invaluable to investigate protein function. However, purity varies in protein batches made in mammalian expression systems, such as CHO-cells or HEK293-cells. This study points to caution while investigating effects of proteins related to the transforming growth factor (TGF)-β superfamily. TGF-β itself is a very potent cytokine and has effects on cells in the femtomolar range. Thus, even very small amounts of contaminating TGF-β in purified protein batches may influence the experimental results given that receptors for TGF-β are present. When we attempted to characterize possible receptors for the TGF-β superfamily ligand GDF15, striking similarities between GDF15-induced activities and known TGF-β activities were found. However, differences between batches of GDF15 were a concern and finally led us to the conclusion that the measured effects were caused by TGF-β and not by GDF15. Our results emphasize that purified recombinant proteins must be used with caution and warrant proper controls. Notably, some conclusions made about GDF15 in already published papers may not be supported by the results shown. Awareness about this issue in the scientific community may prevent spreading of false positive results.nb_NO
dc.language.isoengnb_NO
dc.publisherPublic Library of Sciencenb_NO
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleTGF-β contamination of purified recombinant GDF15nb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.source.volume12nb_NO
dc.source.journalPLoS ONEnb_NO
dc.source.issue11nb_NO
dc.identifier.doi10.1371/journal.pone.0187349
dc.identifier.cristin1517007
dc.description.localcode© 2017 Olsen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.nb_NO
cristin.unitcode194,65,15,0
cristin.unitnameInstitutt for klinisk og molekylær medisin
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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