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dc.contributor.authorTveten, Ann-Kristin
dc.contributor.authorSjåstad, Knut Kåre
dc.date.accessioned2017-11-09T08:36:16Z
dc.date.available2017-11-09T08:36:16Z
dc.date.created2011-09-19T12:18:54Z
dc.date.issued2011
dc.identifier.issn1530-3667
dc.identifier.urihttp://hdl.handle.net/11250/2465096
dc.description.abstractTicks harbor a complex microbial population, which they acquire while feeding on a variety of mammalians and birds. Zoonotic diseases transferred by ticks are an increasing problem and have become a burden to the community. 16S rDNA amplification and denaturing gradient gel electrophoresis (DGGE) enables detection of the broad spectrum of bacteria that settles in the ticks. Profiling the complete microbial population in ticks may provide a better understanding of the ticks' potential to harbor and disperse pathogens. Separation of pathogenic species by DGGE is based on variation in %GC content within the 16S rDNA genetic region. Sequencing of these fragments allows identification of bacterial species. Present study identified some well-known tick-infecting bacteria, such as members of genus Borrelia, Rickettsiales, and Pseudomonas, but also less described tick-infecting bacteria such as Rhodococcus erythropolis, Spiroplasma spp., and an endosymbiont of the microarthropod Folsomia candida. This is the first report of Segniliparus rugosus–infected Ixodes ricinus ticks. Also, it is the first report of several of these pathogens in the Norwegian tick population.nb_NO
dc.language.isoengnb_NO
dc.publisherMary Ann Liebertnb_NO
dc.titleIdentification of Bacteria Infecting Ixodes ricinus Ticks by 16S rDNA Amplification and Denaturing Gradient Gel Electrophoresisnb_NO
dc.typeJournal articlenb_NO
dc.description.versionsubmittedVersionnb_NO
dc.source.journalVector Borne and Zoonotic Diseasesnb_NO
dc.identifier.doi10.1089/vbz.2011.0657
dc.identifier.cristin840294
dc.description.localcodeThis is a submitted manuscript of an article published byMary Ann Liebert for Society for Zoonotic Ecology and Epidemiology (SocZEE) in Vector-Borne and Zoonotic Diseases, October 5, 2011nb_NO
cristin.unitcode194,19,44,0
cristin.unitnameAvdeling for biologiske fag
cristin.ispublishedtrue
cristin.fulltextpreprint
cristin.qualitycode1


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