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dc.contributor.authorDalheim, Marianne Øksnes
dc.contributor.authorVanacker, Julie
dc.contributor.authorNajmi, Maryam A.
dc.contributor.authorAachmann, Finn Lillelund
dc.contributor.authorStrand, Berit Løkensgard
dc.contributor.authorChristensen, Bjørn E.
dc.date.accessioned2017-10-30T12:05:33Z
dc.date.available2017-10-30T12:05:33Z
dc.date.created2015-12-18T08:17:24Z
dc.date.issued2016
dc.identifier.citationBiomaterials. 2016, 80 146-156.nb_NO
dc.identifier.issn0142-9612
dc.identifier.urihttp://hdl.handle.net/11250/2462862
dc.description.abstractPeptide coupled alginates obtained by chemical functionalization of alginates are commonly used as scaffold materials for cells in regenerative medicine and tissue engineering. We here present an alternative to the commonly used carbodiimide chemistry, using partial periodate oxidation followed by reductive amination. High and precise degrees of substitution were obtained with high reproducibility, and without formation of by-products. A protocol was established using l-Tyrosine methyl ester as a model compound and the non-toxic pic-BH3 as the reducing agent. DOSY was used to indirectly verify covalent binding and the structure of the product was further elucidated using NMR spectroscopy. The coupling efficiency was to some extent dependent on alginate composition, being most efficient on mannuronan. Three different bioactive peptide sequences (GRGDYP, GRGDSP and KHIFSDDSSE) were coupled to 8% periodate oxidized alginate resulting in degrees of substitution between 3.9 and 6.9%. Cell adhesion studies of mouse myoblasts (C2C12) and human dental stem cells (RP89) to gels containing various amounts of GRGDSP coupled alginate demonstrated the bioactivity of the material where RP89 cells needed higher peptide concentrations to adhere.nb_NO
dc.language.isoengnb_NO
dc.publisherElseviernb_NO
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.no*
dc.titleEfficient functionalization of alginate biomaterialsnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionacceptedVersionnb_NO
dc.source.pagenumber146-156nb_NO
dc.source.volume80nb_NO
dc.source.journalBiomaterialsnb_NO
dc.identifier.doi10.1016/j.biomaterials.2015.11.043
dc.identifier.cristin1302369
dc.relation.projectNorges forskningsråd: 221576nb_NO
dc.description.localcode© 2015. This is the authors’ accepted and refereed manuscript to the article. LOCKED until 2.12.2017 due to copyright restrictions. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/nb_NO
cristin.unitcode194,66,15,0
cristin.unitnameInstitutt for bioteknologi og matvitenskap
cristin.ispublishedtrue
cristin.fulltextpostprint
cristin.qualitycode2


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Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal