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dc.contributor.authorArlov, Øystein
dc.contributor.authorÖztürk, E.
dc.contributor.authorSteinwachs, M.
dc.contributor.authorSkjåk-Bræk, Gudmund
dc.contributor.authorZenobi-Wong, M.
dc.date.accessioned2017-09-19T14:37:06Z
dc.date.available2017-09-19T14:37:06Z
dc.date.created2017-05-16T15:44:20Z
dc.date.issued2017
dc.identifier.citationEuropean Cells and Materials. 2017, 33 76-89.nb_NO
dc.identifier.issn1473-2262
dc.identifier.urihttp://hdl.handle.net/11250/2455606
dc.description.abstractLoss of articular cartilage from ageing, injury or degenerative disease is commonly associated with inflammation, causing pain and accelerating degradation of the cartilage matrix. Sulphated glycosaminoglycans (GAGs) are involved in the regulation of immune responses in vivo, and analogous polysaccharides are currently being evaluated for tissue engineering matrices to form a biomimetic environment promoting tissue growth while suppressing inflammatory and catabolic activities. Here, we characterise physical properties of sulphated alginate (S-Alg) gels for use in cartilage engineering scaffolds, and study their anti-inflammatory effects on encapsulated chondrocytes stimulated with IL-1β. Sulphation resulted in decreased storage modulus and increased swelling of alginate gels, whereas mixing highly sulphated alginate with unmodified alginate resulted in improved mechanical properties compared to gels from pure S-Alg. S-Alg gels showed extensive anti-inflammatory and anti-catabolic effects on encapsulated chondrocytes induced by IL-1β. Cytokine-stimulated gene expression of pro-inflammatory markers IL-6, IL-8, COX-2 and aggrecanase ADAMTS-5 were significantly lower in the sulphated gels compared to unmodified alginate gels. Moreover, sulphation of the microenvironment suppressed the protein expression of COX-2 and NF-κB as well as the activation of NF-κB and p38-MAPK. The sulphated alginate matrices were found to interact with IL-1β, and proposed to inhibit inflammatory induction by sequestering cytokines from their receptors. This study shows promising potential for sulphated alginates in biomimetic tissue engineering scaffolds, by reducing cytokine-mediated inflammation and providing a protective microenvironment for encapsulated cells.nb_NO
dc.language.isoengnb_NO
dc.publisherAO Research Institute Davosnb_NO
dc.subjectAlginate gelsnb_NO
dc.subjectSulphated alginatenb_NO
dc.subjectCartilage regenerationnb_NO
dc.subjectHuman chondrocytesnb_NO
dc.subjectInflammationnb_NO
dc.titleBiomimetic sulphated alginate hydrogels suppress IL-1B-induced inflammatory responses in human chondrocytesnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.source.pagenumber76-89nb_NO
dc.source.volume33nb_NO
dc.source.journalEuropean Cells and Materialsnb_NO
dc.identifier.doi10.22203/eCM.v033a06
dc.identifier.cristin1470597
dc.relation.projectNorges forskningsråd: 221576nb_NO
dc.description.localcode(c) Authors. This is an Open Access journalnb_NO
cristin.unitcode194,66,15,0
cristin.unitnameInstitutt for bioteknologi
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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