Vis enkel innførsel

dc.contributor.authorGravastrand, Caroline
dc.contributor.authorHamad, Shamal
dc.contributor.authorFure, Hilde
dc.contributor.authorSteinkjer, Bjørg
dc.contributor.authorRyan, Liv
dc.contributor.authorOberholzer, José
dc.contributor.authorLambris, John D.
dc.contributor.authorLacík, Igor
dc.contributor.authorMollnes, Tom Eirik
dc.contributor.authorBrekke, Ole-Lars
dc.contributor.authorRokstad, Anne Mari
dc.date.accessioned2017-06-21T11:46:47Z
dc.date.available2017-06-21T11:46:47Z
dc.date.created2017-06-16T09:35:07Z
dc.date.issued2017
dc.identifier.issn1742-7061
dc.identifier.urihttp://hdl.handle.net/11250/2446581
dc.description.abstractAlginate microspheres are presently under evaluation for future cell-based therapy. Their ability to induce harmful host reactions needs to be identified for developing the most suitable devices and efficient prevention strategies. We used a lepirudin based human whole blood model to investigate the coagulation potentials of alginate-based microspheres: alginate microbeads (Ca/Ba Beads), alginate poly-l-lysine microcapsules (APA and AP microcapsules) and sodium alginate-sodium cellulose sulfate-poly(methylene-co-cyanoguanidine) microcapsules (PMCG microcapsules). Coagulation activation measured by prothrombin fragments 1 + 2 (PTF1.2) was rapidly and markedly induced by the PMCG microcapsules, delayed and lower induced by the APA and AP microcapsules, and not induced by the Ca/Ba Beads. Monocytes tissue factor (TF) expression was similarly activated by the microcapsules, whereas not by the Ca/Ba Beads. PMCG microcapsules-induced PTF1.2 was abolished by FXII inhibition (corn trypsin inhibitor), thus pointing to activation through the contact pathway. PTF1.2 induced by the AP and APA microcapsules was inhibited by anti-TF antibody, pointing to a TF driven coagulation. The TF induced coagulation was inhibited by the complement inhibitors compstatin (C3 inhibition) and eculizumab (C5 inhibition), revealing a complement-coagulation cross-talk. This is the first study on the coagulation potentials of alginate microspheres, and identifies differences in activation potential, pathways and possible intervention points.nb_NO
dc.language.isoengnb_NO
dc.publisherElseviernb_NO
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.no*
dc.titleAlginate microbeads are coagulation compatible, while alginate microcapsules activate coagulation secondary to complement or directly through FXIInb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionacceptedVersionnb_NO
dc.source.journalActa Biomaterialianb_NO
dc.identifier.doihttps://doi.org/10.1016/j.actbio.2017.05.052
dc.identifier.cristin1476564
dc.description.localcode© 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. This is the authors' accepted and refereed manuscript to the article. Locked until 30 May 2019 due to copyright restrictionsnb_NO
cristin.unitcode194,65,15,0
cristin.unitnameInstitutt for kreftforskning og molekylær medisin
cristin.ispublishedfalse
cristin.fulltextpostprint
cristin.qualitycode1


Tilhørende fil(er)

Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel

Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
Med mindre annet er angitt, så er denne innførselen lisensiert som Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal