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dc.contributor.authorSchaart, Jan G.
dc.contributor.authorKjellsen, Trygve Devold
dc.contributor.authorMehli, Lisbeth
dc.contributor.authorHeggem, Reidun
dc.contributor.authorIversen, Tor-Henning
dc.contributor.authorSchouten, Henk J.
dc.contributor.authorKrens, Frans A.
dc.date.accessioned2016-02-18T13:15:28Z
dc.date.accessioned2016-04-27T11:51:51Z
dc.date.available2016-02-18T13:15:28Z
dc.date.available2016-04-27T11:51:51Z
dc.date.issued2011
dc.identifier.citationGenes, Genomes and Genomics 2011(5):102-107nb_NO
dc.identifier.issn1749-0383
dc.identifier.urihttp://hdl.handle.net/11250/2387607
dc.description.abstractThis manuscript discusses different aspects that are relevant to genetically modified strawberry plants with improved characteristics and ‘acceptable’ to consumers and growers of strawberry. It starts with a consumer acceptance survey, held in Norway, Denmark and the UK, studying public perception of genetic modification in general and specifically of genetically modified strawberries with altered properties. This study revealed that genetically modified plants are better accepted by consumers if only genes from the species itself are used for the genetic modification. Subsequently, the results of a functional analysis of the strawberry polygalacturonase inhibiting protein gene (FaPGIP) are described. This indicates that this gene is a possible candidate to induce resistance to Botrytis cinerea when upregulated in strawberry fruits. For this analysis, the FaPGIP gene was overexpressed in transgenic strawberry plants using the cauliflower mosaic virus 35S (CaMV35S) promoter. This showed that FaPGIP overexpression led to resistance to Botrytis in transgenic leaves. For the generation of intragenic (i.e. genetically modification using native genetic elements only) strawberry plants, a transformation vector was constructed in which FaPGIP was combined with a strawberry fruit-specific promoter and terminator that were isolated from a strawberry expansin gene (FaExp2). This vector also included elements that allow the elimination of (foreign) selectable marker genes after genetically modified plant lines have been established. Using this vector, genetically modified strawberry plants were produced that contained only genes from the species itself, and therefore these plants were called intragenic, rather than transgenic. Unfortunately, further evaluations of the intragenic strawberry plants could not demonstrate any enhanced level of resistance to Botrytis in fruits.nb_NO
dc.language.isoengnb_NO
dc.publisherGlobal Science Booksnb_NO
dc.titleTowards the production of genetically modified strawberries which are acceptable to consumersnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.date.updated2016-02-18T13:15:27Z
dc.source.pagenumber102-107nb_NO
dc.source.journalGenes, Genomes and Genomicsnb_NO
dc.source.issue5nb_NO
dc.identifier.cristin870939
dc.description.localcode© Global Science Booksnb_NO


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