dc.contributor.author | Arnfinnsdottir, Nina Bjørk | |
dc.contributor.author | Ottesen, Vegar | |
dc.contributor.author | Lale, Rahmi | |
dc.contributor.author | Sletmoen, Marit | |
dc.date.accessioned | 2015-08-20T07:56:40Z | |
dc.date.accessioned | 2016-04-22T13:43:42Z | |
dc.date.available | 2015-08-20T07:56:40Z | |
dc.date.available | 2016-04-22T13:43:42Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | PLoS ONE 2015, 10(6) | nb_NO |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/11250/2387054 | |
dc.description.abstract | In this paper we demonstrate a procedure for preparing bacterial arrays that is fast, easy,
and applicable in a standard molecular biology laboratory. Microcontact printing is used to
deposit chemicals promoting bacterial adherence in predefined positions on glass surfaces
coated with polymers known for their resistance to bacterial adhesion. Highly ordered arrays
of immobilized bacteria were obtained using microcontact printed islands of polydopamine
(PD) on glass surfaces coated with the antiadhesive polymer polyethylene glycol (PEG). On
such PEG-coated glass surfaces, bacteria were attached to 97 to 100% of the PD islands,
21 to 62% of which were occupied by a single bacterium. A viability test revealed that 99%
of the bacteria were alive following immobilization onto patterned surfaces. Time series imaging
of bacteria on such arrays revealed that the attached bacteria both divided and expressed
green fluorescent protein, both of which indicates that this method of patterning of
bacteria is a suitable method for single-cell analysis. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Public Library of Science | nb_NO |
dc.rights | Navngivelse 3.0 Norge | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/no/ | * |
dc.title | The Design of Simple Bacterial Microarrays: Development towards Immobilizing Single Living Bacteria on Predefined Micro-Sized Spots on Patterned Surfaces | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.date.updated | 2015-08-20T07:56:40Z | |
dc.source.volume | 10 | nb_NO |
dc.source.journal | PLoS ONE | nb_NO |
dc.source.issue | 6 | nb_NO |
dc.identifier.doi | 10.1371/journal.pone.0128162 | |
dc.identifier.cristin | 1247557 | |
dc.description.localcode | © 2015 Arnfinnsdottir et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | nb_NO |