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Nanoparticle Mobility in Pig Small Intestine Mucus (PSIM) - Optimization of Methods used to study Diffusion of Nanoparticles through PSIM, and the Effect of G-block

Wien, Ingrid
Master thesis
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12157_FULLTEXT.pdf (7.656Mb)
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http://hdl.handle.net/11250/2384821
Utgivelsesdato
2015
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  • Institutt for bioteknologi og matvitenskap [886]
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In this thesis, methods for studying diffusion of nanoparticles through pig small intestine mucus (PSIM) were to be studied and optimized. The nanoparticles used were different types of FluoSpheres® and also rhodamine labelled SNEDDS. The project was part of the work package 4 in the Collaboration on the Optimization of Macromolecular Pharmaceutical Access to Cellular Targets (COMPACT) project. By using the optimized methods diffusion through PSIM was observed both with and without the use of G-block together with the nanoparticles to study the effect of G-block on the diffusion. Both fluorometer and confocal microscopy was used to study the diffusion.
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