dc.contributor.author | Zeitlin, SG | |
dc.contributor.author | Chapados, BR | |
dc.contributor.author | Baker, NM | |
dc.contributor.author | Tai, C | |
dc.contributor.author | Slupphaug, Geir | |
dc.contributor.author | Wang, JYJ | |
dc.date.accessioned | 2015-10-30T08:05:58Z | |
dc.date.accessioned | 2015-11-26T12:34:04Z | |
dc.date.available | 2015-10-30T08:05:58Z | |
dc.date.available | 2015-11-26T12:34:04Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | PLoS ONE 2011, 6(3) | nb_NO |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/11250/2365865 | |
dc.description.abstract | Uracil is removed from DNA by the conserved enzyme Uracil DNA N-glycosylase (UNG). Previously, we observed that inhibiting UNG in Xenopus egg extracts blocked assembly of CENP-A, a histone H3 variant. CENP-A is an essential protein in all species, since it is required for chromosome segregation during mitosis. Thus, the implication of UNG in CENP-A assembly implies that UNG would also be essential, but UNG mutants lacking catalytic activity are viable in all species. In this paper, we present evidence that UNG2 colocalizes with CENP-A and H2AX phosphorylation at centromeres in normally cycling cells. Reduction of UNG2 in human cells blocks CENP-A assembly, and results in reduced cell proliferation, associated with increased frequencies of mitotic abnormalities and rapid cell death. Overexpression of UNG2 induces high levels of CENP-A assembly in human cells. Using a multiphoton laser approach, we demonstrate that UNG2 is rapidly recruited to sites of DNA damage. Taken together, our data are consistent with a model in which the N-terminus of UNG2 interacts with the active site of the enzyme and with chromatin. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Public Library of Science | nb_NO |
dc.title | Uracil DNA N-Glycosylase Promotes Assembly of Human Centromere Protein A | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | en_GB |
dc.date.updated | 2015-10-30T08:05:58Z | |
dc.source.volume | 6 | nb_NO |
dc.source.journal | PLoS ONE | nb_NO |
dc.source.issue | 3 | nb_NO |
dc.identifier.doi | 10.1371/journal.pone.0017151 | |
dc.identifier.cristin | 828503 | |
dc.description.localcode | © 2011 Zeitlin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | nb_NO |