dc.contributor.author | Pungente, Michael | |
dc.contributor.author | Jubeli, Emile | |
dc.contributor.author | Øpstad, Christer Lorentz | |
dc.contributor.author | Al-Kawaz, Mais | |
dc.contributor.author | Barakat, Nour | |
dc.contributor.author | Ibrahim, Tarek | |
dc.contributor.author | Abdul Khalique, Nada | |
dc.contributor.author | Raju, Liji | |
dc.contributor.author | Jones, Rachel | |
dc.contributor.author | Leopold, Philip | |
dc.contributor.author | Sliwka, Hans-Richard | |
dc.contributor.author | Partali, Vassilia | |
dc.date.accessioned | 2015-09-30T12:29:37Z | |
dc.date.accessioned | 2015-11-05T13:21:05Z | |
dc.date.available | 2015-09-30T12:29:37Z | |
dc.date.available | 2015-11-05T13:21:05Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Molecules 2012, 17(3):3484-3500 | nb_NO |
dc.identifier.issn | 1420-3049 | |
dc.identifier.uri | http://hdl.handle.net/11250/2359461 | |
dc.description.abstract | The success of nucleic acid delivery requires the development of safe and
efficient delivery vectors that overcome cellular barriers for effective transport. Herein we
describe the synthesis of a series of novel, single-chain rigid cationic carotenoid lipids and
a study of their preliminary in vitro siRNA delivery effectiveness and cellular toxicity. The
efficiency of siRNA delivery by the single-chain lipid series was compared with that of
known cationic lipid vectors, 3β-[N-(N',N'-dimethylaminoethane)carbamoyl]-cholesterol
(DC-Chol) and 1,2-dimyristoyl-sn-glyceryl-3-phosphoethanolamine (EPC) as positive
controls. All cationic lipids (controls and single-chain lipids) were co-formulated into
liposomes with the neutral co-lipid, 1,2-dioleolyl-sn-glycerol-3-phosphoethanolamine
(DOPE). Cationic lipid-siRNA complexes of varying (+/−) molar charge ratios were
formulated for delivery into HR5-CL11 cells. Of the five single-chain carotenoid lipids
investigated, lipids 1, 2, 3 and 5 displayed significant knockdown efficiency with
HR5-CL11 cells. In addition, lipid 1 exhibited the lowest levels of cytotoxicity with cell
viability greater than 80% at all (+/−) molar charge ratios studied. This novel, single-chain rigid carotenoid-based cationic lipid represents a new class of transfection vector with
excellent cell tolerance, accompanied with encouraging siRNA delivery efficiency. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | MDPI | nb_NO |
dc.title | Synthesis and preliminary investigations of the siRNA delivery potential of novel, single-chain rigid cationic carotenoid lipids | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | en_GB |
dc.date.updated | 2015-09-30T12:29:37Z | |
dc.source.volume | 17 | nb_NO |
dc.source.journal | Molecules | nb_NO |
dc.source.issue | 3 | nb_NO |
dc.identifier.doi | 10.3390/molecules17033484 | |
dc.identifier.cristin | 916114 | |
dc.description.localcode | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | nb_NO |