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dc.contributor.authorLilledahl, Magnus Borstad
dc.contributor.authorStokke, Bjørn Torger
dc.date.accessioned2015-09-30T07:15:01Z
dc.date.accessioned2015-11-05T12:21:59Z
dc.date.available2015-09-30T07:15:01Z
dc.date.available2015-11-05T12:21:59Z
dc.date.issued2015
dc.identifier.citationFrontiers in Microbiology 2015, 6nb_NO
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/11250/2359447
dc.description.abstractUnderstanding of biology is underpinned by the ability to observe structures at various length scales. This is so in a historical context and is also valid today. Evolution of novel insight often emerges from technological advancement. Recent developments in imaging technologies that is relevant for characterization of extraceullar microbiological polysaccharides are summarized. Emphasis is on scanning probe and optical based techniques since these tools offers imaging capabilities under aqueous conditions more closely resembling the physiological state than other ultramicroscopy imaging techniques. Following the demonstration of the scanning probe microscopy principle, novel operation modes to increase data capture speed toward video rate, exploitation of several cantilever frequencies, and advancement of utilization of specimen mechanical properties as contrast, also including their mode of operation in liquid, have been developed on this platform. Combined with steps in advancing light microscopy with resolution beyond the far field diffraction limit, non-linear methods, and combinations of the various imaging modalities, the potential ultramicroscopy toolbox available for characterization of exopolysaccharides (EPS) are richer than ever. Examples of application of such ultramicroscopy strategies range from imaging of isolated microbial polysaccharides, structures being observed when they are involved in polyelectrolyte complexes, aspects of their enzymatic degradation, and cell surface localization of secreted polysaccharides. These, and other examples, illustrate that the advancement in imaging technologies relevant for EPS characterization supports characterization of structural aspects.nb_NO
dc.language.isoengnb_NO
dc.publisherFrontiersnb_NO
dc.titleNovel imaging technologies for characterization of microbial extracellular polysaccharidesnb_NO
dc.typeJournal articlenb_NO
dc.typePeer revieweden_GB
dc.date.updated2015-09-30T07:15:01Z
dc.source.volume6nb_NO
dc.source.journalFrontiers in Microbiologynb_NO
dc.identifier.doi10.3389/fmicb.2015.00525
dc.identifier.cristin1258615
dc.description.localcode© 2015 Lilledahl and Stokke. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.nb_NO


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