Analysis and comparision of Transcription Factor - Target Gene databases using network analysis. - The gene regulatory network documented on the basis of experimental evidence is analysed and compared by the graph based analysis method of various resources of gene regulatory network available from various public resource database is performed.
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- Institutt for biologi 
Gene regulatory network assembly and analysis requires high quality knowledge sources that cover functional aspect of various components of the gene regulatory machinery. Transcription factor constitutes key gene regulatory components that usually participate in large multiprotein-DNA complexes, where they guide RNA polymerase (RNAP 1, 2 and 3) activity and regulate the onset and rate of RNA synthesis. A transcription factor (a subclass of which is formed by sequence-specific DNA binding factor) is a protein that binds directly or indirectly to specific DNA regulatory sequences, thereby controlling the flow (or transcription) of genetic information from DNA to messenger RNA Transcription factors perform this function alone or more often together with other proteins in a complex, by promoting (as an activator), or blocking (as a repressor) the recruitment of RNA polymerase (the enzyme that performs the transcription of genetic information from DNA to RNA) to specific genesA defining feature of DNA binding transcription factors is that they contain one or more DNA binding domains (DBDs), which attach to specific sequences of DNA adjacent to the genes that they regulate. Additional proteins such as activators, chromatin remodelers, histone acetylases, deacetylases, kinases, and methylase s, while also playing crucial roles in gene regulation, lacking DNA-binding domains, are not classified as transcription factors. A DNA-binding domain (DBD) is an independently folded protein domain that contains at least one motif that recognizes double- or single-stranded DNA. A DBD can recognize a specific DNA sequence (a recognition sequence) or have a general affinity to DNA. Some DNA-binding domains may also include nucleic acids in their folded structure. However there are various resources - existing with information about mammalian transcription factors. One of the more reliable among them is TFcheckpoint; a database on Transcription factor with strict reference to the experimental evidence that supports both specific binding to the DNA and specific regulation of transcription. This resource provides a comprehensive and high quality knowledge source for genome scale regulatory network studies.Another is HTRIdb which is an open access database of experimentally validated interaction among human transcription factor and their target genes. HTRIdb, however is largely based on the ChIP sequence data.