Blar i Institutt for bioteknologi og matvitenskap på emneord "Bioteknologi (5 årig), Molekylærbiologi"
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Advancements in Construction of XylS/Pm Expression Vectors in Gram-positive Bacteria - Evaluated in Bacillus subtilis and Lactobacillus plantarum
(Master thesis, 2018)Escherichia coli (E. coli) is the most commonly used host for expression of recombinant proteins. A large fraction of recombinant proteins produced in E. coli are stored in an insoluble form, making ... -
Biochemical Characterizations of Enzymes from Oil Reservoir Metagenomes
(Master thesis, 2014)Microorganisms have evolved to exist in diverse environments on earth, even under the harsh conditions of oil reservoirs several kilometres below the seafloor. The environmental conditions there are extreme in several ... -
Capacity of a flow-through biofilter system to secure K-selection and microbial stability in the out-flowing water
(Master thesis, 2018)Water treatment and design of aquaculture systems are typically focused on optimising physicochemical water quality and efforts to maintain bacteria numbers low. High bacteria numbers are not necessarily problematic for ... -
Characterisation of a novel gelatin-based system for oral delivery of pharmaceuticals and nutraceuticals
(Master thesis, 2015)Abstract Oral delivery is one of the most utilized routes for pharmaceutical delivery, due to its simplicity, convenience and thoroughly evaluated safety-profile. Significant drawbacks are however associated with ... -
Characterization of a Paxx Knockout Mouse Model - Growth and immune system development in mice deficient of DNA repair factor PAXX
(Master thesis, 2018)Non-Homologous End-Joining (NHEJ) is the major DNA double-strand break (DSB) repair pathway in higher eukaryotes, functional throughout the cell cycle. NHEJ involves a variety of proteins; some are essential, whereas others ... -
Characterization of peptide fractions from salmon (Salmo salar) rest raw materials towards identifying biologically active fractions
(Master thesis, 2018)Over 150 million tons of fish are produced globally each year of which 25% are discarded. The 25% contain huge amounts of protein rich fish rest raw materials. These proteins can be isolated via enzymatic hydrolysis, which ... -
Conjugative DNA transfer between bacteria and the heterokont alga Nannochloropsis
(Master thesis, 2018)The heterokont microalgae Nannochloropsis have been the subject of many studies that focus on genomic and metabolic engineering. Research on this genus has been motivated by the algae s high-level production of lipids and ... -
Developing a Counterselection System to Facilitate Genetic Modification of Synechococcus sp. PCC 7002. - Construction of counterselection cassettes comprised of sacB and antibiotic resistance genes.
(Master thesis, 2016)Cyanobacteria have recently gained a great deal of attention as hosts for metabolic engineering, due to their innate ability to convert CO2 and sunlight into a variety of chemical products. Cyanobacteria are of special ... -
Development of Protocols for the Extraction, Separation and Characterization of Lipids - Determination of photosynthetic Lipid Composition under different Light Environments in the Microalga Nannochloropsis oceanica
(Master thesis, 2015)The main aim of this research was to develop protocols and techniques for simultaneous extraction, separation and characterization of photosynthetic lipids synthesized in Nannochloropsis oceanica. The lipid composition can ... -
Effects of Hypoxia on Cell Growth and Metabolism in the Leukemia Cell Line JJN-3 and Bladder Cancer Cell Line UM-UC-3
(Master thesis, 2018)Hypoxia is a condition in which the oxygen (O2) access is extremely reduced and thus limits the O2 available for cellular respiration. The hypoxic condition induces metabolic adjustments in the cells that permit further ... -
Effects of Salmon Lice Treatment on Bacterial Density and Community Composition of the Atlantic Salmon Skin Mucus Microbiota
(Master thesis, 2018)The salmon lice (Lepeophtheirus salmonis) infesting Atlantic salmon (Salmo salar) in the on-growing sea water phase is one of the major issues in the Norwegian aquaculture industry. To obtain health and welfare of the ... -
Effects of Sec-Pathway Modifications on Periplasmatic Translocation of Recombinant Proteins in Escherichia coli
(Master thesis, 2018)Recombinant proteins are expressed for use in both research, industry and medicine. The Gram-negative bacteria Escherichia coli (E. coli) is often used as an expression host. It has several properties making it a suitable ... -
Elucidating binding capacities of different sialic acid-carrying polymer substrates with Siglec-9 by Optical Tweezers - Studies of the importance of mucin glycosylation for cancer progression
(Master thesis, 2018)The experimental data regarding interaction studies and energy landscape presented in this master thesis were obtained using a dual-beam Optical Tweezers. The main objective of this study was to investigate the binding of ... -
Elucidating glycan-mediated interactions at cell surfaces by optical tweezers
(Master thesis, 2017)In this study, carbohydrate-interactions were investigated by using optical tweezers, which is an instrument for the measurement of forces down to 0.1 pN. Carbohydrates are added to various biological polymers in a process ... -
Epigenetic changes in human bronchial epithelial cells after in vitro transformation with chemical carcinogens - Analysis of cell migration and DNA methylation in FOXA genes
(Master thesis, 2013)Lung cancer is the leading cause of cancer related deaths worldwide. Cancer is caused by an accumulation of genetic and epigenetic changes that leads to alterations in gene activity and phenotype. There is emerging evidence ... -
Establishing new XylS/Pm based Gram-Negative Protein Production Strains - Evaluating Pseudoalteromonas haloplanktis and Pseudomonas putida
(Master thesis, 2018)To date, Escherichia coli is the most used bacterial host for recombinant protein production. Some proteins are, however, hard to express in this species due to a reducing intracellular environment and inclusion body ... -
Exploring the interaction capacities of microorganisms by the use of high-resolution experimental tools
(Master thesis, 2018)Cells of Saccharomyces cerevisiae poses a possible detoxification method for the removal of mycotoxins through the formation of a yeast-mycotoxin complex. One of the main fungal genus that produces mycotoxins is the Fusarium, ... -
Functional Ingredients in Food
(Master thesis, 2017)In Norway there are more than 4.3 million laying hens that produce an average of 6 eggs every week between the age of five to twelve months. After twelve months the egg production starts to decrease, and when the hens are ... -
Influence of Genetic Background and Environmental Factors on the Skin and Gut Microbiota of Atlantic salmon (Salmo salar) Fry
(Master thesis, 2017)All fish live in symbiosis with complex microbial communities, and the gastrointestinal system (GI) is a natural environment for microbes. The GI microbiota is numerous and diverse, and has important functions for the host. ... -
Intragenomic Variation Among 16S rRNA Copies in Vibrio - Significance of Lifestyle
(Master thesis, 2017)Intragenomic heterogeneity among 16S rRNA gene copies has been found in several species of bacteria. In this thesis, the presence of different 16S rRNA gene copies and the differences in the relative abundance of these 16S ...