Browsing NTNU Open by Author "Zwick, Friederike"
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Exploring the limits of the xylS/Pm expression cassette for recombinant protein production in Escherichia coli:
Zwick, Friederike (Doktoravhandlinger ved NTNU, 1503-8181; 2013:229, Doctoral thesis, 2013)Recombinant protein production is applied for a wide range of applications, however, so far no universal system exists which allows for production of sufficient amounts of any protein of interest. Thus improvement of ... -
Regulation of the expression level of transcription factor XylS reveals new functional insight into its induction mechanism at the Pm promoter
Zwick, Friederike; Lale, Rahmi; Valla, Svein (Journal article; Peer reviewed, 2013)Background XylS is the positive regulator of the inducible Pm promoter, originating from Pseudomonas putida, where the system controls a biochemical pathway involved in degradation of aromatic hydrocarbons, which also act ... -
Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette
Zwick, Friederike; Lale, Rahmi; Valla, Svein (Journal article; Peer reviewed, 2012)The XylS/Pm expression system has been used to produce recombinant proteins at industrial levels in Escherichia coli. Activation of transcription from the Pm promoter takes place in the presence of benzoic acid or derivatives ...