Browsing NTNU Open by Author "Valla, Svein"
Now showing items 1-20 of 28
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A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli
Balzer, Simone; Kucharova, Veronika; Megerle, Judith; Lale, Rahmi; Brautaset, Trygve; Valla, Svein (Journal article; Peer reviewed, 2013)Abstract Background Production of recombinant proteins in bacteria for academic and commercial purposes is a well established field; however the outcomes of process developments for specific proteins are still often ... -
A new and improved host-independent plasmid system for RK2-based conjugal transfer
Aakvik, Trine; Lale, Rahmi; Degnes, Kristin Fløgstad; Lando, Malin; Valla, Svein (Journal article; Peer reviewed, 2014)Bacterial conjugation is a process that is mediated either by a direct cell-to-cell junction or by formation of a bridge between the cells. It is often used to transfer DNA constructs designed in Escherichia coli to recipient ... -
Alginate Biosynthesis Factories in Pseudomonas fluorescens : Localization and Correlation with Alginate Production Level
Maleki, Susan; Almaas, Eivind; Zotchev, Sergey; Valla, Svein; Ertesvåg, Helga (Journal article; Peer reviewed, 2016)Pseudomonas fluorescens is able to produce the medically and industrially important exopolysaccharide alginate. The proteins involved in alginate biosynthesis and secretion form a multiprotein complex spanning the inner ... -
Benzoic Acid-Inducible Gene Expression in Mycobacteria
Dragset, Marte Singsås; Barczak, Amy K.; Kannan, Nisha; Mærk, Mali; Flo, Trude Helen; Valla, Svein; Rubin, Eric J.; Steigedal, Magnus (Journal article; Peer reviewed, 2015)Conditional expression is a powerful tool to investigate the role of bacterial genes. Here, we adapt the Pseudomonas putida-derived positively regulated XylS/Pm expression system to control inducible gene expression in ... -
Bioinformatisk analyse og genetisk modifisering av karbohydratmetabolismen i Pseudomonas fluorescens, med fokus på alginatsyntese fra glukose
Mærk, Mali (Master thesis, 2006)Den sentrale karbohydratmetabolismen i Pseudomonas fluorescens, med vekt på opptak og metabolisme av glukose, ble forsøkt kartlagt ved litteraturstudier og bioinformatisk analyse av tilgjengelige genomsekvenser. Det ble ... -
Cloning and Characterization of Three Transketolase Genes (tktA1, tktA2 and tktA3) from Azotobacter vinelandii
Khider, Miriam (Master thesis, 2010)Alginate is synthesized by the gram-negative bacteria Azotobacter vinelandii. Alginate appears as an important component in both active vegetative cells and in cysts, which is one of the bacteriums life cycle forms. Transketolase ... -
Effekten av å aktivere en genklynge med potensiell kitin syntase aktivitet i Erwinia carotovora
Hagen, Kjersti Næss (Master thesis, 2011)Kitin er en langkjedet, nitrogenholdig polymer som er bygget opp av N-acetyl-glukosamin-enheter (GlcNAc), og finnes i blant annet det ytre skallet til leddyr, og i celleveggen til sopp. Biosyntese av kitin er katalysert ... -
Expression of recombinant proteins in Escherichia coli: The influence of the nucleotide sequences at the 5´ ends of target genes.
Kucharova, Veronika (Doctoral Theses at NTNU, 1503-8181; 287, Doctoral thesis, 2012)The nucleotide sequence at the 5´ end of genes can be specified as the sequence of a promoter associated 5´ untranslated region (UTR) together with the initial coding sequence of a gene. Because this genetic region has ... -
Gel-based proteomics as a tool for increased recovery of extra heavy crude oil
Grebstad, Leni Lødøen (Master thesis, 2011)Two-thirds of the world s remaining oil reserves are made up of heavy and extra heavy crude oils (EHOs). EHOs have a high viscosity due to their complex chemical structure. Production, transport and refining of these are ... -
Identification of genes affecting alginate biosynthesis in Pseudomonas fluorescens by screening a transposon insertion library.
Ertesvåg, Helga; Sletta, Håvard; Senneset, Mona; Sun, Yi-Qian; Klinkenberg, Geir; Konradsen, Therese Aursand; Ellingsen, Trond E; Valla, Svein (Journal article; Peer reviewed, 2017)BACKGROUND: Polysaccharides often are necessary components of bacterial biofilms and capsules. Production of these biopolymers constitutes a drain on key components in the central carbon metabolism, but so far little is ... -
Identification of Regulatory Genes and Metabolic Processes Important for Alginate Biosynthesis in Azotobacter vinelandii by Screening of a Transposon Insertion Mutant Library
Mærk, Mali; Jakobsen, Øyvind Mejdell; Sletta, Håvard; Klinkenberg, Geir; Tøndervik, Anne; Ellingsen, Trond Erling; Valla, Svein; Ertesvåg, Helga (Journal article; Peer reviewed, 2020)Azotobacter vinelandii produces the biopolymer alginate, which has a wide range of industrial and pharmaceutical applications. A random transposon insertion mutant library was constructed from A. vinelandii ATCC12518Tc in ... -
Karakterisering av gener som påvirker biosyntesen av alginat i Pseudomonas fluorescens
Selsaas, Eirik (Master thesis, 2011)Pseudomonas fluorescens har evne til å produsere store store mengder alginat. Tidligere er det laget en stamme, P. fluorescens SBW25 MS1 ΔalgC::TnKb61, hvor algD-operonet og algC er satt under kontroll av Pm-promotor. Dette ... -
Mannuronan C-5 Epimerases Suited for Tailoring of Specific Alginate Structures Obtained by High-Throughput Screening of an Epimerase Mutant Library
Tøndervik, Anne; Klinkenberg, Geir; Aachmann, Finn Lillelund; Svanem, Britt Iren Glærum; Ertesvåg, Helga; Ellingsen, Trond Erling; Valla, Svein; Skjåk-Bræk, Gudmund; Sletta, Håvard (Journal article; Peer reviewed, 2013)The polysaccharide alginate is produced by brown algae and some bacteria and is composed of the two monomers, β-d-mannuronic acid (M) and α-l-guluronic acid (G). The distribution and composition of M/G are important for ... -
Mapping global effects of the anti-sigma factor MucA in Pseudomonas fluorescens SBW25 through genome-scale metabolic modeling
Borgos, Sven Even F.; Bordel, Sergio; Sletta, Håvard; Ertesvåg, Helga; Jakobsen, Øyvind Mejdell; Bruheim, Per; Ellingsen, Trond Erling; Nielsen, Jens; Valla, Svein (Journal article; Peer reviewed, 2013)Background: Alginate is an industrially important polysaccharide, currently produced commercially by harvesting of marine brown sea-weeds. The polymer is also synthesized as an exo-polysaccharide by bacteria belonging to ... -
Meeting Report: 1st International Functional Metagenomics Workshop May 7-8, 2012, St. Jacobs, Ontario, Canada
Engel, Katja; Ashby, Deborah; Brady, Sean F.; Cowan, Don A.; Doemer, John; Edwards, Elizabeth A.; Fiebig, Klaus; Martens, Erik C.; McCormac, Dennis; Mead, David A.; Miyazaki, Kentaro; Moreno-Hagelsieb, Gabriel; O'Gara, Fergal; Reid, Alexandra; Rose, David R.; Simonet, Pascal; Sjöling, Sara; Smalla, Kornelia; Streit, Wolfgang R.; Tedman-Jones, Jennifer; Valla, Svein; Wellington, Elizabeth M.H.; Wu, Cheng-Cang; Liles, Mark R.; Neufeld, Joseph D.; Sessitsch, Angela; Charles, Trevor C. (Journal article; Peer reviewed, 2013)This report summarizes the events of the 1st International Functional Metagenomics Workshop. The workshop was held on May 7 and 8, 2012, in St. Jacobs, Ontario, Canada and was focused on building an international functional ... -
Multilocus sequence typing of Clostridium botulinum
Aas, Anette Kjoshagen (Master thesis, 2011)The construction of the genotyping method Multilocus Sequence Typing (MLST) for the bacteria Clostridium botulinum -
Non-Invasive Analysis of Recombinant mRNA Stability in Escherichia coli by a Combination of Transcriptional Inducer Wash-Out and qRT-PCR
Kucharova, Veronika; Aakvik, Trine; Almaas, Eivind; Naas, Adrian Ertsås; Brautaset, Trygve; Valla, Svein (Journal article; Peer reviewed, 2013)mRNA stability is one among many parameters that can potentially affect the level of recombinant gene expression in bacteria. Blocking of the entire prokaryotic transcription machinery by addition of rifampicin is commonly ... -
Optimzing the 5'-end of Coding Sequences in Recombinant mRNA to achieve high-level Expression in the Bacterium Escherichia coli
Naas, Adrian Ertsås (Master thesis, 2012)Recombinant protein production in Escherichia coli provides a cheap and efficient way of producing medically and industrially relevant proteins. Sequence features of individual genes and especially their 5 terminal coding ... -
Psychrobacter spp. from an Arctic marine environment: Versatile candidates for the degradation of phenolic compounds
Shojaei-Moghadam, Morteza (Doctoral thesis at NTNU;2016:133, Doctoral thesis, 2016)Phenol and its derivatives have challenged pollution/waste management due to being structurally recalcitrant to degradation processes. Bio-based processes continue to be the preferred choice for removal of phenols due to ... -
Recombinant Gene Expression in Escherichia coli: An Experiment and Literature-Based Study of the Roles of the 5'-Ends of Target Genes
Lorentzen, Jon Andreas (Master thesis, 2012)Experiment-based study: The 5 -untranslated region (5 -UTR), and the DNA region corresponding to it, have been shown to have a significant influence on the expression of genes both at transcriptional and translational ...