Blar i NTNU Open på forfatter "Sparstad, Torfinn"
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A CRISPR/Cas9 system adapted for gene editing in marine algae
Sparstad, Torfinn; Sharma, Amit Kumar; Bones, Atle M.; Nymark, Marianne; Winge, Per (Journal article; Peer reviewed, 2016)Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens ... -
Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination - Polyphenol oxidase (PPOA) as biomarker
Moradi, Babak; Kissen, Ralph; Maivan, Hassan Zare; Hashtroudi, Mehri Seyed; Sorahinobar, Mona; Sparstad, Torfinn; Bones, Atle M. (Peer reviewed; Journal article, 2020)Mangrove plants, which inhabit and form sensitive ecosystems in the intertidal zones of tropical and subtropical coastlines, though vulnerable to petroleum pollution, still maintain their growth under oil contamination. ... -
Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination – Polyphenol oxidase (PPOA) as a biomarker
Moradi, Babak; Kissen, Ralph; Maivan, Hassan Zare; Hashtroudi, Mehri Seyed; Sorahinobar, Mona; Sparstad, Torfinn; Bones, Atle M. (Journal article; Peer reviewed, 2020) -
Expression of DNA repair genes in arctic char (Salvelinus alpinus) from Bjørnøya in the Norwegian Arctic
Inderberg, Helene; Neerland, Eirik D.; Mcpartland, Molly; Sparstad, Torfinn; Bytingsvik, Jenny; Nikiforov, Vladimir A; Evenset, Anita; Krøkje, Åse (Peer reviewed; Journal article, 2021)High levels of organochlorines (OCs) have been measured in arctic char (Salvelinus alpinus) from Lake Ellasjøen on Bjørnøya, Norway (74.30°N, 19.0°E). In a nearby lake, Laksvatn, the OC-levels in arctic char were low. A ... -
Simultaneous knockout of multiple LHCF genes using single sgRNAs and engineering of a high-fidelity Cas9 for precise genome editing in marine algae
Sharma, Amit Kumar; Nymark, Marianne; Flo, Snorre; Sparstad, Torfinn; Bones, Atle M.; Winge, Per (Peer reviewed; Journal article, 2021)The CRISPR/Cas9 system is an RNA-guided sequence-specific genome editing tool, which has been adopted for single or multiple gene editing in a wide range of organisms. When working with gene families with functional ... -
Transgene-free genome editing in marine algae by bacterial conjugation - comparison with biolistic CRISPR/Cas9 transformation
Sharma, Amit Kumar; Nymark, Marianne; Sparstad, Torfinn; Bones, Atle M.; Winge, Per (Journal article; Peer reviewed, 2018)The CRISPR/Cas9 technology has opened the possibility for targeted genome editing in various organisms including diatom model organisms. One standard method for delivery of vectors to diatom cells is by biolistic particle ...