Browsing NTNU Open by Author "Sletmoen, Marit"
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Adhesion of common salmonid pathogens to Atlantic salmon mucins
Tonstad, Johanna (Master thesis, 2020)Fisk er dekket av slimhinner, mucus, på alle ytre overflateepitel, som utgjør den første forsvarslinjen mot farlige organismer. Skal et patogen infisere en fisk, må det først passere gjennom denne barrieren i skinnet, ... -
Bacterial Microarrays by Microcontact Printing: Development of a Method for Immobilizing Live Bacteria on Microarrays
Ottesen, Vegar (Master thesis, 2014)Traditional microbiological experimental methods generally reveal population-wide statistics and are not capable of revealing variations between individual cells. In an effort to address this, a cheap, quick and easy means ... -
Bakteriell adhesjon til mukosale overflater - Effektar av mikrobiota på bakteriell adhesjon til lakseskinn
Erdal, Magnhild Sekse (Master thesis, 2019)Fisk lever i direkte kontakt med sitt ytre miljø, og er difor kontinuerleg utsette for eksterne truslar. Skinnet er den fyrste forsvarslinja mot det ytre miljøet, og det er dekt med eit slimlag, også kalla mucus. Mucus ... -
Bruk av nanobioteknologiverktøy for å studere mekanismar som ligg til grunn for bakteriell adhesjon til mucin
Ølnes, Åsa Schawlann (Master thesis, 2019)Nokre av dagens største helseproblem, inkludert overvekt, diabetes og allergiar, er påverka av tarmfloraen. Koloniseringa av kommensale bakteriar til vert, samt infeksjonar forårsaka av patogene bakteriar, avhenger begge ... -
Characterization of Substrate Binding and Hybrid Forming Abilities of Galectin-3
Kjær, Sylvi Oliva (Master thesis, 2018)Glycans have over the last decade become a hot topic in biochemistry, cell biology and related research areas. Glycoproteins are today known to have critical roles in the organization and function of eukaryotic cells. Their ... -
Design and characterisation of patterned surfaces for bacterial microarray fabrication
Krigul, Kertu Liis (Master thesis, 2018)The main objective of this master project was to prepare master moulds and PDMS stamps which can be used for microcontact printing. In addition, the aim was to develop and characterize patterned surfaces which can be used ... -
Direct Determination of Chitosan–Mucin Interactions Using a Single-Molecule Strategy: Comparison to Alginate–Mucin Interactions
Haugstad, Kristin Elisabeth; Håti, Armend Gazmeno; Nordgård, Catherine Taylor; Adl, Patricia S.; Maurstad, Gjertrud; Sletmoen, Marit; Draget, Kurt Ingar; Dias, Rita de Sousa; Stokke, Bjørn Torger (Journal article; Peer reviewed, 2015)Aqueous chitosan possesses attractive interaction capacities with various molecular groups that can be involved in hydrogen bonds and electrostatic and hydrophobic interactions. In the present paper, we report on the direct ... -
Effect of design geometry, exposure energy, cytophilic molecules, cell type and load in fabrication of single-cell arrays using micro-contact printing
Bhujbal, Swapnil Vilas; Dekov, Maren; Ottesen, Vegar; Dunker, Karen; Lale, Rahmi; Sletmoen, Marit (Journal article; Peer reviewed, 2020)In this study a range of factors influencing the fabrication of single-cell arrays (SCAs) are identified and investigated. Micro-contact printing was used to introduce spots coated with polyethyleneimine or Matrigel on ... -
Elucidating binding capacities of different sialic acid-carrying polymer substrates with Siglec-9 by Optical Tweezers - Studies of the importance of mucin glycosylation for cancer progression
Monshaugen, Ida (Master thesis, 2018)The experimental data regarding interaction studies and energy landscape presented in this master thesis were obtained using a dual-beam Optical Tweezers. The main objective of this study was to investigate the binding of ... -
Elucidating glycan-mediated interactions at cell surfaces by optical tweezers
Siarpilina, Katsiaryna (Master thesis, 2017)In this study, carbohydrate-interactions were investigated by using optical tweezers, which is an instrument for the measurement of forces down to 0.1 pN. Carbohydrates are added to various biological polymers in a process ... -
Energy Landscape of Alginate-Epimerase Interactions Assessed by Optical Tweezers and Atomic Force Microscopy
Håti, Armend Gazmeno; Aachmann, Finn Lillelund; Stokke, Bjørn Torger; Skjåk-Bræk, Gudmund; Sletmoen, Marit (Journal article, 2015)Mannuronan C-5 epimerases are a family of enzymes that catalyze epimerization of alginates at the polymer level. This group of enzymes thus enables the tailor-making of various alginate residue sequences to attain various ... -
Exploring the interaction abilities of Galectin-3 using the sensitive force probe optical tweezers
Aamlid, Katarina Stiff (Master thesis, 2020)Glykaner har den unike evnen til å vise og lagre et høyt innhold av kompleks biologisk informasjon, noe som gjør dem essensielle for flere cellulære aktiviteter. Kompleksiteten forbundet med glykanstrukturer har likevel ... -
Exploring the interaction capacities of microorganisms by the use of high-resolution experimental tools
Bay, Lilli Theres Eilertsen (Master thesis, 2018)Cells of Saccharomyces cerevisiae poses a possible detoxification method for the removal of mycotoxins through the formation of a yeast-mycotoxin complex. One of the main fungal genus that produces mycotoxins is the Fusarium, ... -
Fabrication of Cellular Microarrays
Resell, Aurora (Master thesis, 2017)Cellular microarrays are known for their use as a tool to investigate variations between single cells in terms of how they respond to different physiological parameters. The work of this thesis focused on developing a ... -
Immobilisation of E. coli by Microarray Printing.
Shrestha, Retina (Master thesis, 2018)Cellular heterogeneity is a fundamental property of organisms that help them to adapt and thrive in different changing environmental conditions. Therefore, approaches are necessary to study cellular processes at a single ... -
Immobilisering av Saccharomyces cerevisiae og virkning av kitosan på den mekaniske styrken til celleveggen til Saccharomyces cerevisiae
Samseth, Åshild (Master thesis, 2016)Målet for denne masteroppgaven var å utvikle en metode for immobilisering av S. cerevisiae til en glassoverflate samt å undersøke hvordan celleveggens elastisitet til S. cerevisiae blir påvirket av kitosan med 66% eller ... -
Interactions between the breast cancer-associated MUC1 mucins and C-type lectin characterized by optical tweezers
Hadjialirezaei, Soosan; Picco, Gianfranco; Beatson, Richard; Burchell, Joy; Stokke, Bjørn Torger; Sletmoen, Marit (Journal article; Peer reviewed, 2017)Carbohydrate–protein interactions govern many crucial processes in biological systems including cell recognition events. We have used the sensitive force probe optical tweezers to quantify the interactions occurring between ... -
Interaksjoner mellom galektiner og deres substrater
Wik, Viktoria Rosfjord (Master thesis, 2019)Glykoforskning representerer et nytt og spennende forskningsfelt som handler om å forstå funksjonen og strukturen til glykaner, og det gir håp om spennende utvikling innen områder som medisin, energigenerering og ... -
Microarrays for High Throughput Analysis of Cellular Heterogeneity
Dunker, Karen (Master thesis, 2017)The importance of cellular heterogeneity within cell populations has been increasingly realized in recent years. As a result, there has been a focus on developing methods for single cell analysis. µCP represents a cheap, ... -
Microarrays for the study of compartmentalized microorganisms in alginate microbeads and (W/O/W) double emulsions
Håti, Armend Gazmeno; Arnfinnsdottir, Nina Bjørk; Østevold, Camilla; Sletmoen, Marit; Etienne, Gianluca; amstad, esther; Stokke, Bjørn Torger (Journal article; Peer reviewed, 2016)Understanding of cells has mainly been achieved through analysis of average properties of large cellular populations. The increased awareness of cellular heterogeneity in various biological systems, spans from single ...