Browsing NTNU Open by Author "Sikorski, Pawel"
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A correlative spatiotemporal microscale study of calcium phosphate formation and transformation within an alginate hydrogel matrix.
Bassett, David; Ucar, Seniz; Sikorski, Pawel; Andreassen, Jens-Petter; Bjørnøy, Sindre Hove; Strand, Berit Løkensgard (Journal article; Peer reviewed, 2016)The modification of soft hydrogels with hard inorganic components is a method used to form composite materials with application in non-load-bearing bone tissue engineering. The inclusion of an inorganic component may provide ... -
Analysis of Actin and Focal Adhesion Organisation in U2OS Cells on Polymer Nanostructures
Vinje, Jakob; Guadagno, Noemi Antonella; Progida, Cinzia; Sikorski, Pawel (Peer reviewed; Journal article, 2021)Background In this work, we explore how U2OS cells are affected by arrays of polymer nanopillars fabricated on flat glass surfaces. We focus on describing changes to the organisation of the actin cytoskeleton and in the ... -
Application of Temporary, Cell-Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels
Lehnert, Sarah; Sikorski, Pawel (Journal article; Peer reviewed, 2021)Mimicking the complexity of natural tissue is a major challenge in the field of tissue engineering. Here, a facile 2-step fabrication method to prepare 3D constructs with distinct regions of high cell concentrations and ... -
Bio-instructive materials for musculoskeletal regeneration
Gonzalez-Fernandez, Tomas; Sikorski, Pawel; Leach, J. Kent (Journal article; Peer reviewed, 2019)The prevalence and cost of disorders affecting the musculoskeletal system are predicted to rise significantly in the coming years due to the aging global population and the increase of associated risk factors. Despite being ... -
Biochemical and Structural Characterization of Neocartilage Formed by Mesenchymal Stem Cells in Alginate Hydrogels
Magnus Østgård, Olderøy; Lilledahl, Magnus Borstad; Beckwith, Marianne; Melvik, Jan Egil; Reinholt, Finn P.; Sikorski, Pawel; Brinchmann, Jan E. (Journal article; Peer reviewed, 2014)A popular approach to make neocartilage in vitro is to immobilize cells with chondrogenic potential in hydrogels. However, functional cartilage cannot be obtained by control of cells only, as function of cartilage is largely ... -
Biocomposites prepared by alkaline phosphatase mediated mineralization of alginate microbeads
Xie, Minli; Magnus Østgård, Olderøy; Zhang, Zhibing; Andreassen, Jens-Petter; Strand, Berit Løkensgard; Sikorski, Pawel (Journal article; Peer reviewed, 2012)This study set out to develop a biomimetic scaffold by incorporating osteoinductive hydroxyapatite (HA) particles into a porous alginate gel matrix via a cell-friendly pathway. Two types of alginate/calcium phosphate ... -
Calcite seed-assisted microbial induced carbonate precipitation (MICP)
Zehner, Jennifer Simone; Røyne, Anja; Sikorski, Pawel (Peer reviewed; Journal article, 2021)Microbial-induced calcium carbonate precipitation (MICP) is a biological process inducing biomineralization of CaCO3. This can be used to form a solid, concrete-like material. To be able to use MICP successfully to produce ... -
Competitive ligand exchange of crosslinking ions for ionotropic hydrogel formation.
Bassett, David; Håti, Armend Gazmeno; Melø, Thor Bernt; Stokke, Bjørn Torger; Sikorski, Pawel (Journal article; Peer reviewed, 2016)Currently there are limitations to gelation strategies to form ionically crosslinked hydrogels, derived in particular from a lack of control over the release kinetics of crosslinking ions, which severely restrict applications. ... -
Controlled mineralisation and recrystallisation of brushite within alginate hydrogels
Bjørnøy, Sindre Hove; Bassett, David; Ucar, Seniz; Sikorski, Pawel; Andreassen, Jens-Petter (Journal article; Peer reviewed, 2016-02)Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration ... -
Developing 2.5D Nanotopographical in vitro Neural Network Recording Platform to Mimic the Physical Environment of the Brain
Isdal, Leik Bjelland (Master thesis, 2021)På tross av sin vanvittige kompleksitet er nervesystemet en del av menneskets anatomi som er spesielt sårbar. Nevrodegenerative sykdommer som Alzheimers og Parkinsons forårsaker omfattende og uopprettelig skade hos de som ... -
Development of a tailored microfluidic platform with improved electrical signal detection for in vitro studies of neural networks and neural network pathology
Winter-Hjelm, Nicolai (Master thesis, 2020)Menneskets nervesystem utgjør verdens mest avanserte evolusjonære maskineri. Gjennom dets høyst intrikate arkitektur og beregningsevner kontrollerer det oppgaver så funksjonelt forskjellige som muskelbevegelser og lagring ... -
Dissolution of copper mineral phases in biological fluids and the controlled release of copper ions from mineralized alginate hydrogels
Bassett, David; Madzovska, Ivana; Beckwith, Kai Sandvold; Melø, Thor Bernt; Sikorski, Pawel; Obradovic, Bojana (Journal article; Peer reviewed, 2015)Here we investigate the dissolution behaviour of copper minerals contained within biocompatible alginate hydrogels. Copper has a number of biological effects and has most recently been evaluated as an alternative to expensive ... -
Effects of Nanostructured SU-8 Surfaces on Neurons and Neural Networks in Vitro
Hvide, Edevard (Master thesis, 2021)Nanotopografiske vekstflater har de siste årene blitt anvendt som en lovende strategi for å øke topologisk kompleksitet av biologiske nevrale nettverk dyrket på in vitro forskningsplattformer. Slike plattformer kan ... -
Electroconductive scaffolds for tissue engineering applications
Sikorski, Pawel (Journal article, 2020)Many material systems that can conduct electronic current have been in recent years studied in the context of tissue engineering. It is suggested that materials that can carry electronic current are necessary or beneficial ... -
Electron Beam Lithography Fabrication of SU-8 Polymer Structures for Cell Studies
Vinje, Jakob; Beckwith, Kai Sandvold; Sikorski, Pawel (Journal article; Peer reviewed, 2020)Flat surfaces decorated with micro- and nanostructures are important tools in biomedical research used to control cellular shape, in studies of mechanotransduction, membrane mechanics, cell migration and cellular interactions ... -
Formation of Hydroxyapatite via Transformation of Amorphous Calcium Phosphate in the Presence of Alginate Additives
Ucar, Seniz; Bjørnøy, Sindre Hove; Bassett, David; Strand, Berit Løkensgard; Sikorski, Pawel; Andreassen, Jens-Petter (Journal article; Peer reviewed, 2019)Hydroxyapatite (HA) is the primary mineral of vertebral tooth and bone tissue; thus, it is often incorporated into synthetic composite materials designed for hard tissue engineering applications. Understanding the formation ... -
Gelling kinetics and in situ mineralization of alginate hydrogels: A correlative spatiotemporal characterization toolbox.
Åslund, Andreas; Bassett, David; Ucar, Seniz; Sikorski, Pawel; Andreassen, Jens-Petter; Mandaric, Stefan; Bjørnøy, Sindre Hove; Strand, Berit Løkensgard (Journal article; Peer reviewed, 2016)Due to their large water content and structural similarities to the extracellular matrix, hydrogels are an attractive class of material in the tissue engineering field. Polymers capable of ionotropic gelation are of special ... -
Influence of Nanopillar Arrays on Fibroblast Motility, Adhesion, and Migration Mechanisms
Beckwith, Kai Sandvold; Ullmann, Sindre; Vinje, Jakob; Sikorski, Pawel (Journal article; Peer reviewed, 2019)Surfaces decorated with high aspect ratio nanostructures are a promising tool to study cellular processes and design novel devices to control cellular behavior. However, little is known about the dynamics of cellular ... -
Local structure of Ca2+ alginate hydrogels gelled via competitive ligand exchange and measured by small angle X-ray scattering
Yamamoto, K; Yuguchi, Yoshiaki; Stokke, Bjørn Torger; Sikorski, Pawel; Bassett, David (Journal article; Peer reviewed, 2019)Alginates, being linear anionic co-polymers of 1,4-linked residues β-d-ManA (M) and α-l-GulA (G), are widely applied as hydrogel biomaterials due to their favourable in vivo biocompatibility and convenient ionic crosslinking. ... -
Micro-scale studies of microbialinduced carbonate precipitation for the application of biocementation
Zehner, Jennifer (Doctoral theses at NTNU;2021:59, Doctoral thesis, 2021)Cement, which is used as a binding material in conventional concrete, is the second most consumed material by humans, after water. The production process of cement, however, causes a high emission of CO2 and contributes ...