Browsing NTNU Open by Author "Sharma, Animesh"

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    • A Novel Truncated Form of Nephronectin Is Present in Small Extracellular Vesicles Isolated from 66cl4 Cells 

      Toraskar, Jimita Prashant; Norvoll Magnussen, Synnøve; Hagen, Lars; Sharma, Animesh; Hoang, Linh; Bjørkøy, Geir; Svineng, Gunbjørg; Steigedal, Tonje S. (Journal article; Peer reviewed, 2019)
      Extracellular vesicles are emerging as biomarkers in breast cancer. Our recent report suggested that an intracellular granular staining pattern of the extracellular matrix protein nephronectin (NPNT) in breast tumor sections ...

    • Absence of NLRP3 Inflammasome in Hematopoietic Cells Reduces Adverse Remodeling After Experimental Myocardial Infarction 

      Louwe, Maria Cornelia; Olsen, Maria Belland; Kaasbøll, Ole Jørgen; Yang, Kuan; Fosshaug, Linn Elisabeth Lillerud; Alfsnes, Katrine; Øgaard, Jonas; Rashidi, Azita; Skulberg, Vidar Magne; Yang, Mingyi; Fonseca, Davi de Miranda; Sharma, Animesh; Aronsen, Jan Magnus; Schrumpf, Elisabeth; Ahmed, Muhammad Shakil; Dahl, Christen Peder; Nyman, Tuula Anneli; Ueland, Thor; Melum, Espen; Halvorsen, Bente; Bjørås, Magnar; Attramadal, Håvard; Sjaastad, Ivar; Aukrust, Pål; Yndestad, Arne (Peer reviewed; Journal article, 2020)
      An inflammatory response is required for tissue healing after a myocardial infarction (MI), but the process must be balanced to prevent maladaptive remodeling. This study shows that improved survival and cardiac function ...

    • ALKBH3 partner ASCC3 mediates P-body formation and selective clearance of MMS-induced 1-methyladenosine and 3-methylcytosine from mRNA 

      Wollen, Kristian Lied; Hagen, Lars; Vågbø, Cathrine Broberg; Rabe, Renana; Iveland, Tobias Solli; Aas, Per Arne; Sharma, Animesh; Sporsheim, Bjørnar; Erlandsen, Hilde O.; Palibrk, Vuk; Bjørås, Magnar; Fonseca, Davi de Miranda; Mosammaparast, Nima; Slupphaug, Geir (Peer reviewed; Journal article, 2021)
      Background: Reversible enzymatic methylation of mammalian mRNA is widespread and serves crucial regulatory functions, but little is known to what degree chemical alkylators mediate overlapping modifcations and whether cells ...

    • Changes in cellular signaling proteins in extracts from A549, H460, and U2OS cells treated with cisplatin or docetaxel 

      Petrovic, Voin; Olaisen, Camilla; Sharma, Animesh; Nepal, Anala; Bugge, Steffen; Sundby, Eirik; Hoff, Bård Helge; Slupphaug, Geir; Otterlei, Marit (Journal article; Peer reviewed, 2017)
      Cell extracts from A549, H460, and U2OS human cancer cell lines treated with cisplatin and docetaxel were analyzed by mass spectrometry (MS) proteomic analysis. The extracts were enriched for cellular signaling proteins ...

    • Comparative Proteomic Analysis Reveals Varying Impact on Immune Responses in Phorbol 12-Myristate-13-Acetate-Mediated THP-1 Monocyte-to-Macrophage Differentiation 

      Pinto, Sneha M.; Kim, Hera; Subbannayya, Yashwanth; Giambelluca, Miriam S.; Bösl, Korbinian; Ryan, Liv; Sharma, Animesh; Kandasamy, Richard Kumaran (Journal article; Peer reviewed, 2021)
      Macrophages are sentinels of the innate immune system, and the human monocytic cell line THP-1 is one of the widely used in vitro models to study inflammatory processes and immune responses. Several monocyte-to-macrophage ...

    • Differential regulation of cysteine oxidative post-translational modifications in high and low aerobic capacity 

      Souza, Rodrigo W. A.; Alves, Christiano R. R.; Medeiros, Alessandra; Rolim, Natale Pinheiro Lage; Silva, Gustavo Jose Justo; Moreira, Jose Bianco Nascimento; Alves, Marcia Netto Magalhaes; Wohlwend, Martin; Gebriel, Mohammed; Hagen, Lars; Sharma, Animesh; Koch, Lauren G.; Britton, Steven L.; Slupphaug, Geir; Wisløff, Ulrik; Brum, Patricia C. (Journal article; Peer reviewed, 2018)
      Given the association between high aerobic capacity and the prevention of metabolic diseases, elucidating the mechanisms by which high aerobic capacity regulates whole-body metabolic homeostasis is a major research challenge. ...

    • Exercise training reverses cancer-induced oxidative stress and decrease in muscle COPS2/TRIP15/ALIEN 

      Alves, Christiano R. R.; des Neves, Willian; de Almeida, Ney R; Eichelberger, Eric J; Jannig, Paulo R.; Voltarelli, Vanessa A; Tobias, Gabriel C; Bechara, Luiz R G; de Paula Faria, Daniele; Alves, Maria J N; Hagen, Lars; Sharma, Animesh; Slupphaug, Geir; Moreira, Jose Bianco Nascimento; Wisløff, Ulrik; Hirshman, Michael F.; Negrao, Carlos E.; de Castro Jr, Gilberto; Chammas, Roger; Swoboda, Kathryn J.; Ruas, Jorge L.; Goodyear, Laurie J.; Brum, Patricia Chakur (Peer reviewed; Journal article, 2020)
      Objective We tested the hypothesis that exercise training would attenuate metabolic impairment in a model of severe cancer cachexia. Methods We used multiple in vivo and in vitro methods to explore the mechanisms ...

    • HDACi Mediate UNG2 Depletion, Dysregulated Genomic Uracil and Altered Expression of Oncoproteins and Tumor Suppressors in B- And T-cell Lines 

      Iveland, Tobias Solli; Hagen, Lars; Sharma, Animesh; Sousa, Mirta; Sarno, Antonio; Kristian Lied, Wollen; Liabakk, Nina-Beate; Slupphaug, Geir (Peer reviewed; Journal article, 2020)
      Background HDAC inhibitors (HDACi) belong to a new group of chemotherapeutics that are increasingly used in the treatment of lymphocyte-derived malignancies, but their mechanisms of action remain poorly understood. Here ...

    • Long-Term Exposure to Nanosized TiO2 Triggers Stress Responses and Cell Death Pathways in Pulmonary Epithelial Cells 

      Alswady-Hoff, Mayes; Erdem, Johanna Maria Samulin; Phuyal, Santosh; Knittelfelder, Oskar; Sharma, Animesh; Fonseca, Davi de Miranda; Skare, Øivind; Slupphaug, Geir; Zienolddiny, Shanbeh (Peer reviewed; Journal article, 2021)
      There is little in vitro data available on long-term effects of TiO2 exposure. Such data are important for improving the understanding of underlying mechanisms of adverse health effects of TiO2 . Here, we exposed pulmonary ...

    • Loss of CpFTSY Reduces Photosynthetic Performance and Affects Insertion of PsaC of PSI in Diatoms 

      Nymark, Marianne; Finazzi, Giovanni; Volpe, Charlotte; Serif, Manuel Philipp; Fonseca, Davi de Miranda; Sharma, Animesh; Sanchez Puerto, Nicolas; Sharma, Amit Kumar; Ashcroft, Felicity Jayne; Kissen, Ralph; Winge, Per; Bones, Atle Magnar (Peer reviewed; Journal article, 2023)
      The chloroplast signal recognition particle (CpSRP) receptor (CpFTSY) is a component of the CpSRP pathway that post-translationally targets light-harvesting complex proteins (LHCPs) to the thylakoid membranes in plants and ...

    • Modulation of cell metabolic pathways and oxidative stress signaling contribute to acquired melphalan resistance in multiple myeloma cells. 

      Zub, Kamila Anna; Sousa, Mirta; Sarno, Antonio; Sharma, Animesh; Demirovic, Aida; Rao, Shalini; Young, Clifford; Aas, Per Arne; Ericsson, Ida; Sundan, Anders; Jensen, Ole Nørregaard; Slupphaug, Geir (Journal article; Peer reviewed, 2015)
      Alkylating agents are widely used chemotherapeutics in the treatment of many cancers, including leukemia, lymphoma, multiple myeloma, sarcoma, lung, breast and ovarian cancer. Melphalan is the most commonly used chemotherapeutic ...

    • Monitoring of the spatial and temporal dynamics of BER/SSBR pathway proteins, including MYH, UNG2, MPG, NTH1 and NEIL1-3, during DNA replication. 

      Bjørås, Karine; Sousa, Mirta; Sharma, Animesh; Fonseca, Davi de Miranda; Søgaard, Caroline Danielsen; Bjørås, Magnar; Otterlei, Marit (Journal article; Peer reviewed, 2017)
      Base lesions in DNA can stall the replication machinery or induce mutations if bypassed. Consequently, lesions must be repaired before replication or in a post-replicative process to maintain genomic stability. Base excision ...

    • MS-proteomics provides insight into the host responses towards alginate microspheres 

      Coron, Abba Elizabeth; Fonseca, Davi de Miranda; Sharma, Animesh; Slupphaug, Geir; Strand, Berit Løkensgard; Rokstad, Anne Mari A. (Journal article; Peer reviewed, 2022)
      Protein adsorption to biomaterial surfaces is considered a determining factor for the host response. Here we detail the protein adsorption profiles of alginate hydrogel microspheres relevant for cell therapy using mass ...

    • Nucleoside Analogues Are Potent Inducers of Pol V-mediated Mutagenesis 

      Sumabe, Balagra Kasim; Ræder, Synnøve Brandt; Røst, Lisa M.; Sharma, Animesh; Donkor, Eric S.; Mosi, Lydia; Duodu, Samuel; Bruheim, Per; Otterlei, Marit (Peer reviewed; Journal article, 2021)
      Drugs targeting DNA and RNA in mammalian cells or viruses can also affect bacteria present in the host and thereby induce the bacterial SOS system. This has the potential to increase mutagenesis and the development of ...

    • Nucleoside Analogues Are Potent Inducers of Pol V-mediated Mutagenesis 

      Sumabe, Balagra Kasim; Ræder, Synnøve Brandt; Røst, Lisa M.; Sharma, Animesh; Donkor, Eric S.; Mosi, Lydia; Duodu, Samuel; Bruheim, Per; Otterlei, Marit (Journal article; Peer reviewed, 2021)
      Drugs targeting DNA and RNA in mammalian cells or viruses can also affect bacteria present in the host and thereby induce the bacterial SOS system. This has the potential to increase mutagenesis and the development of ...

    • An optimized MALDI MSI protocol for spatial detection of tryptic peptides in fresh frozen prostate tissue 

      Høiem, Therese Stork; Andersen, Maria Karoline; Martin-Lorenzo, Marta; Longuespée, Rémi; Claes, Britt S.R.; Nordborg, Anna; Dewez, Frédéric; Balluff, Benjamin; Giampà , Marco; Sharma, Animesh; Hagen, Lars; Heeren, Ron M.A.; Bathen, Tone Frost; Giskeødegård, Guro F.; Krossa, Sebastian; Tessem, May-Britt (Peer reviewed; Journal article, 2022)
      MALDI MS imaging (MSI) is a powerful analytical tool for spatial peptide detection in heterogeneous tissues. Proper sample preparation is crucial to achieve high quality, reproducible measurements. Here we developed an ...

    • Proteome alterations associated with transformation of multiple myeloma to secondary plasma cell leukemia 

      Zatula, Alexey; Dikic, Aida; Mulder, Teska Celine; Sharma, Animesh; Vågbø, Cathrine Broberg; Sousa, Mirta; Waage, Anders; Slupphaug, Geir (Peer reviewed; Journal article, 2017)
      Plasma cell leukemia is a rare and aggressive plasma cell neoplasm that may either originate de novo (primary PCL) or by leukemic transformation of multiple myeloma (MM) to secondary PCL (sPCL). The prognosis of sPCL is ...

    • Proteomic analysis reveals mechanisms underlying increased efficacy of bleomycin by photochemical internalization in bladder cancer cells 

      Gederaas, Odrun Arna; Sharma, Animesh; Mbarak, Saide; Sporsheim, Bjørnar; Høgset, Anders; Bogoeva, Vanya; Slupphaug, Geir; Hagen, Lars (Peer reviewed; Journal article, 2023)
      Photochemical internalization (PCI) is a promising new technology for site-specific drug delivery, developed from photodynamic therapy (PDT). In PCI, light-induced activation of a photosensitizer trapped inside endosomes ...

    • Targeting the non-canonical roles of PCNA modifies and increases the response to targeted anti-cancer therapy 

      Søgaard, Caroline Krogh; Nepal, Anala; Petrovic, Voin; Sharma, Animesh; Liabakk, Nina-Beate; Steigedal, Tonje S.; Otterlei, Marit (Journal article, 2019)
      Receptor tyrosine kinases (RTKs), such as HER2 and/or EGFR are important therapeutic targets in multiple cancer cells. Low and/or short response to targeted therapies are often due to activation of compensatory signaling ...

    • UDP-glucose dehydrogenase expression is upregulated following EMT and differentially affects intracellular glycerophosphocholine and acetylaspartate levels in breast mesenchymal cell lines 

      Wang, Qiong; Karvelsson, Sigurdur Trausti; Johannsson, Freyr; Vilhjalmsson, Arnar Ingi; Hagen, Lars; Fonseca, Davi de Miranda; Sharma, Animesh; Slupphaug, Geir; Rolfsson, Ottar (Journal article; Peer reviewed, 2021)
      Metabolic rewiring is one of the indispensable drivers of epithelial-mesenchymal transition (EMT) involved in breast cancer metastasis. In this study, we explored the metabolic changes during spontaneous EMT in three ...