Browsing NTNU Open by Author "Kavli, Bodil Merete"
Now showing items 1-7 of 7
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AID expression in B-cell lymphomas causes accumulation of genomic uracil and a distinct AID mutational signature
Pettersen, Henrik Sahlin; Galashevskaya, Anastasia; Doseth, Berit; Sousa, Mirta; Sarno, Antonio; Visnes, Torkild; Aas, Per Arne; Liabakk, Nina-Beate; Slupphaug, Geir; Sætrom, Pål; Kavli, Bodil Merete; Krokan, Hans Einar (Journal article; Peer reviewed, 2014)tThe most common mutations in cancer are C to T transitions, but their origin has remained elusive.Recently, mutational signatures of APOBEC-family cytosine deaminases were identified in many com-mon cancers, suggesting ... -
Backbone 1H, 13C and 15N chemical shift assignment of full-length human uracil DNA glycosylase UNG2
Buchinger, Edith; Wiik, Siv Åshild; Kusnierczyk, Anna; Rabe, Renana; Aas, Per Arne; Kavli, Bodil Merete; Slupphaug, Geir; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2017)Human uracil N-glycosylase isoform 2—UNG2 consists of an N-terminal intrinsically disordered regulatory domain (UNG2 residues 1–92, 9.3 kDa) and a C-terminal structured catalytic domain (UNG2 residues 93–313, 25.1 kDa). ... -
Repair of genomic uracil and ribonucleotides - Regulation and potential involvement in new and old cancer treatments
Iveland, Tobias Solli (Doctoral theses at NTNU;2024:393, Doctoral thesis, 2024)Life as we know it has evolved over millions of years and evolution has produced complex biochemical machineries that exist in every living cell. Many of these processes may appear imperfect and to threaten the stability ... -
Robust DNA repair in PAXX-deficient mammalian cells
Dewan, Alisa Elinsdatter; Xing, Mengtan; Lundbæk, Marie Benner; Gago-Fuentes, Raquel; Beck, Carole; Aas, Per Arne; Liabakk, Nina-Beate; Sæterstad, Siri; Khac Thanh Phong, Chau; Kavli, Bodil Merete; Oksenych, Valentyn (Journal article; Peer reviewed, 2018)To ensure genome stability, mammalian cells employ several DNA repair pathways. Nonhomologous DNA end joining (NHEJ) is the DNA repair process that fixes double-strand breaks throughout the cell cycle. NHEJ is involved in ... -
RPA2 winged-helix domain facilitates UNG-mediated removal of uracil from ssDNA; implications for repair of mutagenic uracil at the replication fork
Kavli, Bodil Merete; Iveland, Tobias Solli; Buchinger, Edith; Hagen, Lars; Liabakk, Nina-Beate; Aas, Per Arne; Obermann, Tobias Sebastian; Aachmann, Finn Lillelund; Slupphaug, Geir (Journal article; Peer reviewed, 2021)Uracil occurs at replication forks via misincorporation of deoxyuridine monophosphate (dUMP) or via deamination of existing cytosines, which occurs 2-3 orders of magnitude faster in ssDNA than in dsDNA and is 100% miscoding. ... -
UNG-initiated base excision repair is the major repair route for 5-fluorouracil in DNA, but 5-fluorouracil cytotoxicity depends mainly on RNA incorporation
Pettersen, Henrik Sahlin; Visnes, Torkild; Vågbø, Cathrine Broberg; Svaasand, Eva K; Doseth, Berit; Slupphaug, Geir; Kavli, Bodil Merete; Krokan, Hans Einar (Journal article; Peer reviewed, 2011)Cytotoxicity of 5-fluorouracil (FU) and 5-fluoro-2′-deoxyuridine (FdUrd) due to DNA fragmentation during DNA repair has been proposed as an alternative to effects from thymidylate synthase (TS) inhibition or RNA incorporation. ... -
Uracil-DNA glycosylase UNG1 isoform variant supports class switch recombination and repairs nuclear genomic uracil
Sarno, Antonio; Lundbæk, Marie Benner; Liabakk, Nina-Beate; Aas, Per Arne; Mjelle, Robin; Hagen, Lars; Sousa, Mirta; Krokan, Hans Einar; Kavli, Bodil Merete (Journal article; Peer reviewed, 2019)UNG is the major uracil-DNA glycosylase in mammalian cells and is involved in both error-free base excision repair of genomic uracil and mutagenic uracil-processing at the antibody genes. However, the regulation of UNG in ...