Browsing NTNU Open by Author "Hohmann-Marriott, Martin Frank"
Now showing items 1-20 of 36
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A Practical Solution for 77 K Fluorescence Measurements Based on LED Excitation and CCD Array Detector
Lamb, Jacob Joseph; Forfang, Kristin; Hohmann-Marriott, Martin Frank (Journal article, 2015)The fluorescence emission spectrum of photosynthetic microorganisms at liquid nitrogen temperature (77 K) provides important insights into the organization of the photosynthetic machinery of bacteria and eukaryotes, which ... -
An adjustable algal chloroplast plug-and-play model for genome-scale metabolic models
Røkke, Gunvor Bjerkelund; Hohmann-Marriott, Martin Frank; Almaas, Eivind (Peer reviewed; Journal article, 2020)The chloroplast is a central part of plant cells, as this is the organelle where the photosynthesis, fixation of inorganic carbon, and other key functions related to fatty acid synthesis and amino acid synthesis occur. ... -
Biochemical Characterizations of Enzymes from Oil Reservoir Metagenomes
Hesthammer, Ronja Kristine (Master thesis, 2014)Microorganisms have evolved to exist in diverse environments on earth, even under the harsh conditions of oil reservoirs several kilometres below the seafloor. The environmental conditions there are extreme in several ... -
Biochemical Characterizations of Enzymes from Oil Reservoir Metagenomes
Hesthammer, Ronja Kristine (Master thesis, 2014)Microorganisms have evolved to exist in diverse environments on earth, even under the harsh conditions of oil reservoirs several kilometres below the seafloor. The environmental conditions there are extreme in several ... -
Changes in Lipid Composition Induced by Environmental and Genetic Manipulation in Synechocystis sp. PCC 6803
Forfang, Kristin (Master thesis, 2014)The objective of this research was to investigate the photosynthetic lipids synthesized by the cyanobacterium Synechocystis sp. PCC 6803 and to develop methods to extract, separate and characterize them. A particular focus ... -
Chlorophyll fluorescence emission spectroscopy of oxygenic organisms at 77 K
Lamb, Jacob Joseph; Røkke, Gunvor; Hohmann-Marriott, Martin Frank (Journal article; Peer reviewed, 2018)Photosynthetic fluorescence emission spectra measurement at the temperature of 77 K (–196°C) is an often-used technique in photosynthesis research. At low temperature, biochemical and physiological processes that modulate ... -
Cloning and Expression of Four Laccase genes isolated from Arctic marine Psychrobacter strains in Escherichia coli and psychrophilic Pseudomonas
Egharevba, Osasumwen (Master thesis, 2017)A group of diverse bacterial isolates were discovered in Arctic marine habitat during a research expedition in May 2009. These isolates were identified and sequenced based on 16S rRNA sequencing. Psychrobacter strains ... -
Conjugative DNA transfer between bacteria and the heterokont alga Nannochloropsis
Khider, May Laura Kilano (Master thesis, 2018)The heterokont microalgae Nannochloropsis have been the subject of many studies that focus on genomic and metabolic engineering. Research on this genus has been motivated by the algae s high-level production of lipids and ... -
Creating a Gene Expression Cassette for the Cyanobacterium Synechococcus sp. PCC 7002 - And Using it to Replace the Endogenous Hydrogenase
Williams, Christopher Wright (Master thesis, 2015)Eukaryotic algae and cyanobacteria are a promising source of future biofuels, having many important advantages over 1st and 2nd generation biofuels. Synechococcus sp. PCC7002 is the fastest growing oxygen producing ... -
Cyanobacterial siderophores - physiology, structure, biosynthesis, and applications
Årstøl, Erland; Hohmann-Marriott, Martin Frank (Journal article; Peer reviewed, 2019)Siderophores are low-molecular-weight metal chelators that function in microbial iron uptake. As iron limits primary productivity in many environments, siderophores are of great ecological importance. Additionally, their ... -
Design, construction and evaluation of protein expression systems in Synechocystis PCC 6803.
Sunar, Binod Gautam (Master thesis, 2014)student will create constructs that are designed to transfer expression system to the chromosome of Synechocystis. Furthermore it will be tested if the expression systemcan also be implemented on the native plasmids of ... -
Developing a Counterselection System to Facilitate Genetic Modification of Synechococcus sp. PCC 7002. - Construction of counterselection cassettes comprised of sacB and antibiotic resistance genes.
Nordstrand, Christina Elen (Master thesis, 2016)Cyanobacteria have recently gained a great deal of attention as hosts for metabolic engineering, due to their innate ability to convert CO2 and sunlight into a variety of chemical products. Cyanobacteria are of special ... -
Developing molecular tools for the genetic manipulation of Nannochloropsis
Chernyavskaya, Oxana (Master thesis, 2014)The main aim of the master project was the establishment of the efficient protocols for the genetic manipulation (nuclear transformation and chloroplast transformation) of Nannochloropsis oceanica CCMP1779. Nannochloropsis ... -
Developing Molecular Tools To Genetically Engineer The Microalga Nannochloropsis
Anley, Kominist Asmamaw (Master thesis, 2015)The main objective of this thesis was to develop an efficient transformation protocol for Nannochloropsis oceanica CCMP1779 by biolistic transformation using the linearized pSELECT100 plasmid which confers hygromycin B ... -
Developing tools to genetically engineer the microalga Nannochloropsis
Nguyen, Vy (Master thesis, 2016)Genetic manipulation is an important tool to engineer organisms for biotechnological applications. While directed gene replacement has been established in many bacteria, only few eukaryotic systems are known to allow ... -
Development of Protocols for the Extraction, Separation and Characterization of Lipids - Determination of photosynthetic Lipid Composition under different Light Environments in the Microalga Nannochloropsis oceanica
Gulyaeva, Ksenia (Master thesis, 2015)The main aim of this research was to develop protocols and techniques for simultaneous extraction, separation and characterization of photosynthetic lipids synthesized in Nannochloropsis oceanica. The lipid composition can ... -
Engineering of Orthogonal Aminoacyl tRNA Synthetase/tRNA Pairs for Codon Reassignment in the Chloroplast of Chlamydomonas reinhardtii
Solbakken, Eirik (Master thesis, 2020)Mikroalger etablerer seg som en attraktiv produksjonsplatform for rekombinante proteiner og andre biobaserte produkter. Kloroplasten opprettholder sitt eget maskineri for proteinsyntese og et kompakt kromosom, som på enkelt ... -
From the Ocean to the Lab—Assessing Iron Limitation in Cyanobacteria: An Interface Paper
Hunnestad, Annie Vera; Vogel, Anne Ilse Maria; Digernes, Maria Guadalupe; Armstrong, Evelyn; Ardelan, Murat Van; Hohmann-Marriott, Martin Frank (Peer reviewed; Journal article, 2020)Iron is an essential, yet scarce, nutrient in marine environments. Phytoplankton, and especially cyanobacteria, have developed a wide range of mechanisms to acquire iron and maintain their iron-rich photosynthetic machinery. ... -
Functional Role of PilA in Iron Acquisition in the Cyanobacterium Synechocystis sp. PCC 6803
Lamb, Jacob Joseph; Hill, Ryan; Eaton-Rye, Julian J.; Hohmann-Marriott, Martin Frank (Journal article; Peer reviewed, 2014)Cyanobacteria require large quantities of iron to maintain their photosynthetic machinery; however, in most environments iron is present in the form of insoluble iron oxides. Whether cyanobacteria can utilize these sources ... -
Genome analysis of four novel Psychrobacter spp. and characterisation of their six putative laccase-like multicopper oxidases using bioinformatics tools
Rise, Kjersti (Master thesis, 2015)In May 2009, several Psychrobacter spp. were found at the bottom of the Norwegian sea outside of Svalbard. Four of these, P11F6, P2G3, P11G3 and P11G5, were selected and sequenced for further work, and form the basis for ...