Browsing NTNU Open by Author "Gawin, Agnieszka Maria"
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Functional characterization of three Azotobacter chroococcum alginate-modifying enzymes related to the Azotobacter vinelandii AlgE mannuronan C-5-epimerase family
Gawin, Agnieszka Maria; Tietze, Lisa; Aarstad, Olav Andreas; Aachmann, Finn Lillelund; Brautaset, Trygve; Ertesvåg, Helga (Peer reviewed; Journal article, 2020)Bacterial alginate initially consists of 1-4-linked beta-D-mannuronic acid residues (M) which can be later epimerized to alpha-L-guluronic acid (G). The family of AlgE mannuronan C-5-epimerases from Azotobacter vinelandii ... -
Translational regulation of periplasmic folding assistants and proteases as a valuable strategy to improve production of translocated recombinant proteins in Escherichia coli
Gawin, Agnieszka Maria; Ertesvåg, Helga; Hartvigsen Hansen, Sine Alise; Malmo, Jostein; Brautaset, Trygve (Peer reviewed; Journal article, 2020)Background Advantages of translocation of recombinant proteins to the periplasm in Escherichia coli include simplified downstream processing, and improved folding and in vivo activity of the target protein. There are, ...