Browsing NTNU Open by Author "Garcia Calvo, Laura"
Now showing items 1-4 of 4
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Central carbon metabolite profiling reveals vector-associated differences in the recombinant protein production host Escherichia coli BL21
Garcia Calvo, Laura; Rane, Divyata Vilas; Everson, Nikalet; Humlebrekk, Sigurd Tømmerberg; Mathiassen, Lise Femanger; Mæhlum, Astfrid Helene Morka; Malmo, Jostein; Bruheim, Per (Peer reviewed; Journal article, 2023)The Gram-negative bacterium Escherichia coli is the most widely used host for recombinant protein production, both as an industrial expression platform and as a model system at laboratory scale. The recombinant protein ... -
Experimental determination of Escherichia coli biomass composition for constraint-based metabolic modeling
Simensen, Vetle; Schulz, Christian; Karlsen, Emil; Bråtelund, Signe; Burgos, Idun Maria Tokvam; Thorfinnsdottir, Lilja Brekke; Garcia Calvo, Laura; Bruheim, Per; Almaas, Eivind (Peer reviewed; Journal article, 2022)Genome-scale metabolic models (GEMs) are mathematical representations of metabolism that allow for in silico simulation of metabolic phenotypes and capabilities. A prerequisite for these predictions is an accurate ... -
First feed matters: The first diet of larval fish programmes growth, survival, and metabolism of larval ballan wrasse (Labrus bergylta)
Malzahn, Arne; Ribicic, Deni; Hansen, Bjørn Henrik; Sarno, Antonio; Kjørsvik, Elin; Aase, Anna Sigrid Nordberg; Alves Musialak, Luciana; Garcia Calvo, Laura; Hagemann, Andreas (Peer reviewed; Journal article, 2022)The use of cleaner fish, such as the ballan wrasse (Labrus bergylta), is important for combatting the sea lice problem in salmonid cage farming. Ballan wrasse is the only wrasse species that is cultivated, though only about ... -
Optimized Fast Filtration-based Sampling and Extraction Enables Precise and Absolute Quantification of the Escherichia coli Central Carbon Metabolome
Thorfinnsdottir, Lilja Brekke; Garcia Calvo, Laura; Bø, Gaute Hovde; Bruheim, Per; Røst, Lisa Marie Ivarsdatter (Peer reviewed; Journal article, 2023)Precise and accurate quantification is a prerequisite for interpretation of targeted metabolomics data, but this task is challenged by the inherent instability of the analytes. The sampling, quenching, extraction, and ...