Browsing NTNU Open by Author "Fuchino, Katsuya"
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Absolute Quantification of the Central Carbon Metabolome in Eight Commonly Applied Prokaryotic and Eukaryotic Model Systems
Røst, Lisa Marie; Thorfinnsdottir, Lilja Brekke; Kumar, Kanhaiya; Fuchino, Katsuya; Langørgen, Ida Eide; Bartosova, Zdenka; Kristiansen, Kåre Andre; Bruheim, Per (Peer reviewed; Journal article, 2020)Absolute quantification of intracellular metabolite pools is a prerequisite for modeling and in-depth biological interpretation of metabolomics data. It is the final step of an elaborate metabolomics workflow, with challenges ... -
An assessment of serial co-cultivation approach for generating novel Zymomonas mobilis strains
Fuchino, Katsuya; Bruheim, Per (Peer reviewed; Journal article, 2020)Objective The alphaproteobacterium Zymomonas mobilis is an efficient ethanol producer, and Z. mobilis-based biorefinery shows great potential for biofuel production. Serial co-cultivation is an emerging approach that ... -
Genome Copy Number Quantification Revealed That the Ethanologenic Alpha-Proteobacterium Zymomonas mobilis Is Polyploid
Fuchino, Katsuya; Wasser, Daniel; Soppa, Jörg (Peer reviewed; Journal article, 2021)The alpha-proteobacterium Zymomonas mobilis is a promising biofuel producer, based on its native metabolism that efficiently converts sugars to ethanol. Therefore, it has a high potential for industrial-scale biofuel ... -
Improvement of acetaldehyde production in Zymomonas mobilis by engineering of its aerobic metabolism
Kalnenieks, Uldis; Balodite, Elina; Strahler, Steffi; Strazdina, Inese; Rex, Julia; Pentjuss, Agris; Fuchino, Katsuya; Bruheim, Per; Rutkis, Reinis; Pappas, Katherine M.; Poole, Robert K.; Sawodny, Oliver; Bettenbrock, Katja (Journal article; Peer reviewed, 2019)Acetaldehyde is a valuable product of microbial biosynthesis, which can be used by the chemical industry as the entry point for production of various commodity chemicals. In ethanologenic microorganisms, like yeast or the ... -
Increased salt tolerance in Zymomonas mobilis strain generated by adaptative evolution
Fuchino, Katsuya; Bruheim, Per (Peer reviewed; Journal article, 2020)Background Ethanologenic alphaproteobacterium Zymomonas mobilis has been acknowledged as a promising biofuel producer. There have been numerous efforts to engineer this species applicable for an industrial-scale bioethanol ... -
Metabolic Profiling of Glucose-Fed Metabolically Active Resting Zymomonas mobilis Strains
Fuchino, Katsuya; Kalnenieks, Uldis; Rutkis, Reinis; Grube, Mara; Bruheim, Per (Peer reviewed; Journal article, 2020)Zymomonas mobilis is the most efficient bacterial ethanol producer and its physiology is potentially applicable to industrial-scale bioethanol production. However, compared to other industrially important microorganisms, ... -
Zwitterionic HILIC tandem mass spectrometry with isotope dilution for rapid, sensitive and robust quantification of pyridine nucleotides in biological extracts
Røst, Lisa Marie; Shafaei, Armaghan; Fuchino, Katsuya; Bruheim, Per (Peer reviewed; Journal article, 2020)The pyridine nucleotides nicotineamide adenine dinucleotide (NAD) and nicotineamide adenine dinucleotide phosphate (NADP) are conserved coenzymes across all domains of life, and are involved in more than 200 different ...