Browsing NTNU Open by Author "Ertesvåg, Helga"
Now showing items 1-20 of 54
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A structural and functional study of AlgE mannuronan C5-epimerases and lyases from Azotobacter vinelandii and Azotobacter chroococcum
Sønsteby, Sigrid Tejera (Master thesis, 2021)Alginat er lineære, anioniske polysakkarider, som består av (1→4) bundet β-D-mannuron- syre (M) og α-L-guluronsyre (G). Hvert polysakkarid syntetiseres først som mannuronan og G-enheter introduseres av mannuronan C5-epimeraser ... -
A study of AlgE-type mannuronan C-5-epimerases from Azotobacter chroococcum and Azotobacter vinelandii
Hervig, Silje Trå (Master thesis, 2022)Alginat er en biopolymer bestående av beta-D-mannuronsyre (M) og alfa-L-guluronsyre (G), bundet sammen med 1-4 glykosidbindinger. Alginat er biokompatibelt og kan danne hydrogeler, som for eksempel kan brukes i medisinsk ... -
A Study of c-di-GMP Levels and Alginate Production in Pseudonomas fluorescens SBW25
Haaland, Helena (Master thesis, 2022)Alginat er en lineær biopolymer med stor industriell og medisinsk verdi, på grunn av sine geleformende egenskaper. Alginat kan danne geler når en av de to monomerene som bygger opp alginat, guluronsyre (G) og danner ... -
A study of the polysaccharide Psl in P. fluorescens by controlling the amount of proteins (putatively) involved in Psl synthesis
Grägg, Camilla Sende (Master thesis, 2023)Polysaccharid synthesis locus (Psl) er et polysakkarid produsert av blant annet Pseudomonas aeruginosa, hvor det består av mannose, rhamnose og glukose. Psl er involvert i dannelsen av biofilm, og har vist seg å være viktig ... -
A study of two potential carnitine acetyltransferase genes and their role in the formation of acetyl-CoA for lipid biosynthesis in Aurantiochytrium limacinum SR21
Holmlund, Ingrid (Master thesis, 2022)Thraustochytrider, inkludert arten A. limacinum, har et potensiale til å akkumulere store mengder lipider under gunstige vekstforhold. For å kunne utnytte mikroorganismene som en kilde til verdifulle n-3 flerumettede ... -
Alginate Biosynthesis Factories in Pseudomonas fluorescens : Localization and Correlation with Alginate Production Level
Maleki, Susan; Almaas, Eivind; Zotchev, Sergey; Valla, Svein; Ertesvåg, Helga (Journal article; Peer reviewed, 2016)Pseudomonas fluorescens is able to produce the medically and industrially important exopolysaccharide alginate. The proteins involved in alginate biosynthesis and secretion form a multiprotein complex spanning the inner ... -
Alginate Biosynthesis in Pseudomonas fluorescens SBW25: Investigating Regulatory Aspects Involving Levansucrase and c-di-GMP
Braadland, Tora Selvåg (Master thesis, 2023)Alginat er en mye brukt polysakkarid i mat- og legemiddelindustrien. Den består av de kovalent bundne monomerene mannuronsyre (M) og, dens epimer, guluronsyre (G). Sammensetningen av monomerene gjør alginat allsidig, hvilket ... -
Alginate-modifying enzymes: Biological roles and biotechnological uses
Ertesvåg, Helga (Journal article; Peer reviewed, 2015)Alginate denotes a group of industrially important 1-4-linked biopolymers composed of the C-5-epimers β-D-mannuronic acid (M) and α-L-guluronic acid (G). The polysaccharide is manufactured from brown algae where it constitutes ... -
Alginates as Green Flocculants for Metal Oxide Nanoparticles
Slabov, Vladislav; Jain, Garima; Chernyshova, Irina; Kota, Hanumantha Rao; Ertesvåg, Helga (Peer reviewed; Journal article, 2023)Flocculation is used for the removal or separation of colloids, e.g. in water treatment and mineral processing. Alginates are linear, anionic biopolymers composed of mannuronic (M) and guluronic (G) acids. The relative ... -
Assessing the Capability of Pseudomonas fluorescens and Pseudomonas savastanoi to Produce G-Rich Alginates
May, Carlan (Master thesis, 2020)Alginate is a naturally occurring polymer produced by brown algae and certain bacterial species – specifically those in the genera Azotobacter and Pseudomonas. This polymer has many applications including as a thickening ... -
ATP-Dependent Citrate Lyase and Glycerol 3-Phosphate Dehydrogenase in Aurantiochytrium T66: An Attempted In Vivo Analysis and Heterologous Expression
Jakobsen, Margit Ertresvåg (Master thesis, 2019)Thraustochytrider er industrielt interessante mikroorganismer på grunn av deres evne til å produsere store mengder lipider, spesielt omega-3 fettsyren dokosaheksaensyre (DHA). Metabolismen til disse organismene er ikke ... -
Combined Metabolome and Lipidome analyses for in-depth characterization of lipid accumulation in the DHA producing Aurantiochytrium sp. T66
Bartosova, Zdenka; Ertesvåg, Helga; Nyfløt, Eirin Lishaugen; Kämpe, Kristoffer; Aasen, Inga Marie; Bruheim, Per (Peer reviewed; Journal article, 2021)Thraustochytrids are marine heterotrophic microorganisms known for their potential to accumulate docosahexaenoic acid (DHA)-enriched lipids. There have been many attempts to improve thraustochytrid DHA bioprocesses, ... -
Constructing a Hybrid De novo Genome Assembly of Aurantiochytrium sp. T66 using MinION and Illumina reads to Improve the Quality of its Annotation
Hamza, Amir (Master thesis, 2021)Thraustochytrids are unicellular marine heterotrophs which have attention due to their ability to accumulate high amount of important nutraceuticals, in particular polyunsaturated fatty acids (PUFA). However, their ... -
Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production
Gawin, Agnieszka; Peebo, Karl; Sebastian, Hans; Ertesvåg, Helga; Irla, Marta Katarzyna; Neubauer, Peter; Brautaset, Trygve (Journal article; Peer reviewed, 2019)Background Bacteria are widely used as hosts for recombinant protein production due to their rapid growth, simple media requirement and ability to produce high yields of correctly folded proteins. Overproduction of ... -
Development and testing of antibiotic resistance and promoter cassettes for a versatile toolbox of conditional suicide plasmids in Marinobacter algicola
Nærby, Tuva Askmann (Master thesis, 2024)Denne oppgaven er en del av en utvikling av en fleksibel verktøykasse for betingede selvmordsplas- mider, hvor hvert plasmid best ̊ar av kassetter av forskjellige egenskaper som enkelt kan byttes ut for ̊a tilpasse ulike ... -
Development and Testing of Promoter Strength and Cassettes for Regulation of Replication with TrfA in Azotobacter chroococcum
Wagle, Vilde Wøien (Master thesis, 2024)Denne studien er en del av et større prosjekt som har som mål å utvikle en verktøykasse for innsetting og fjerning av DNA, designet som et betinget selvmordsplasmid basert på RK2-plasmidet. Fordelen med et betinget ... -
Draft genome sequence of the docosahexaenoic acid producing thraustochytrid Aurantiochytrium sp. T66
Liu, Bin; Ertesvåg, Helga; Aasen, Inga Marie; Vadstein, Olav; Brautaset, Trygve; Heggeset, Tonje Marita Bjerkan (Journal article; Peer reviewed, 2016)Thraustochytrids are unicellular, marine protists, and there is a growing industrial interest in these organisms, particularly because some species, including strains belonging to the genus Aurantiochytrium, accumulate ... -
Effekten av å aktivere en genklynge med potensiell kitin syntase aktivitet i Erwinia carotovora
Hagen, Kjersti Næss (Master thesis, 2011)Kitin er en langkjedet, nitrogenholdig polymer som er bygget opp av N-acetyl-glukosamin-enheter (GlcNAc), og finnes i blant annet det ytre skallet til leddyr, og i celleveggen til sopp. Biosyntese av kitin er katalysert ... -
En studie av funksjonaliteten til tre potensielle diacylglyserol acyltransferaser (DGAT) fra thraustochytriden Aurantiochytrium limacinum SR21
Tjøtta, Berit Rø (Master thesis, 2024)De marine omega-3-fettsyrene er essensielle for mennesker, og et adekvat inntak av disse fettsyrene har flere helsefremmende effekter. Den viktigste kilden til omega-3-fettsyrene er i dag fiske av små pelagisk fisk utenfor ... -
Functional characterization of three Azotobacter chroococcum alginate-modifying enzymes related to the Azotobacter vinelandii AlgE mannuronan C-5-epimerase family
Gawin, Agnieszka Maria; Tietze, Lisa; Aarstad, Olav Andreas; Aachmann, Finn Lillelund; Brautaset, Trygve; Ertesvåg, Helga (Peer reviewed; Journal article, 2020)Bacterial alginate initially consists of 1-4-linked beta-D-mannuronic acid residues (M) which can be later epimerized to alpha-L-guluronic acid (G). The family of AlgE mannuronan C-5-epimerases from Azotobacter vinelandii ...