Browsing NTNU Open by Author "Buchinger, Edith"
Now showing items 1-5 of 5
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Backbone 1H, 13C and 15N chemical shift assignment of full-length human uracil DNA glycosylase UNG2
Buchinger, Edith; Wiik, Siv Åshild; Kusnierczyk, Anna; Rabe, Renana; Aas, Per Arne; Kavli, Bodil Merete; Slupphaug, Geir; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2017)Human uracil N-glycosylase isoform 2—UNG2 consists of an N-terminal intrinsically disordered regulatory domain (UNG2 residues 1–92, 9.3 kDa) and a C-terminal structured catalytic domain (UNG2 residues 93–313, 25.1 kDa). ... -
The effect of linker conformation on performance and stability of a two-domain lytic polysaccharide monooxygenase
Forsberg, Zarah; Stepnov, Anton; Tesei, Giulio; Wang, Yong; Buchinger, Edith; Kristiansen, Sandra; Aachmann, Finn Lillelund; Arleth, Lise; Eijsink, Vincent; Lindorff-Larsen, Kresten; Courtade, Gaston (Journal article; Peer reviewed, 2023)A considerable number of lytic polysaccharide monooxygenases (LPMOs) and other carbohydrate-active enzymes are modular, with catalytic domains being tethered to additional domains, such as carbohydrate-binding modules, by ... -
Functional characterization of a lytic polysaccharide monooxygenase from Schizophyllum commune that degrades non-crystalline substrates
Østby, Heidi; Christensen, Idd Andrea; Hennum, Karen; Varnai, Aniko; Buchinger, Edith; Grandal, Siri; Courtade, Gaston; Hegnar, Olav Aaseth; Aachmann, Finn Lillelund; Eijsink, Vincent (Journal article; Peer reviewed, 2023)Lytic polysaccharide monooxygenases (LPMOs) are mono-copper enzymes that use O2 or H2O2 to oxidatively cleave glycosidic bonds. LPMOs are prevalent in nature, and the functional variation among these enzymes is a topic of ... -
RPA2 winged-helix domain facilitates UNG-mediated removal of uracil from ssDNA; implications for repair of mutagenic uracil at the replication fork
Kavli, Bodil Merete; Iveland, Tobias Solli; Buchinger, Edith; Hagen, Lars; Liabakk, Nina-Beate; Aas, Per Arne; Obermann, Tobias Sebastian; Aachmann, Finn Lillelund; Slupphaug, Geir (Journal article; Peer reviewed, 2021)Uracil occurs at replication forks via misincorporation of deoxyuridine monophosphate (dUMP) or via deamination of existing cytosines, which occurs 2-3 orders of magnitude faster in ssDNA than in dsDNA and is 100% miscoding. ... -
Structural and functional characterization of the R-modules in alginate C-5 epimerases AlgE4 and AlgE6 from Azotobacter vinelandii
Buchinger, Edith; Knudsen, Daniel H.; Behrens, Manja A.; Pedersen, Jan Skov; Aarstad, Olav Andreas; Tøndervik, Anne; Valla, Svein; Skjåk-Bræk, Gudmund; Wimmer, Reinhard; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2014)The bacterium Azotobacter vinelandii produces a family of seven secreted and calcium-dependent mannuronan C-5 epimerases (AlgE1–7). These epimerases are responsible for the epimerization of β-d-mannuronic acid (M) to ...