Browsing NTNU Open by Author "Bruheim, Per"
Now showing items 41-60 of 141
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Exploring the Effects of Adaptation to Serum-Free Cultivation for Adherent and Suspended HEK293 Cells
Pete, Ellen Sofie (Master thesis, 2021)Cellekultur er en samlebetegnelse på dyrking av celler utenfor organismen de opprinner fra. Dette er et felt med stor vitenskapelig og industriell relevans, blant annet for produksjon av biofarmasøytika og vaksiner, og for ... -
Exploring the metabolic adaptations in CHO-K1 cells cultured in serum free media and the effect of adaptation method utilized
Renjo, Marietta Mellemløkken (Master thesis, 2021)Serum er en flytende forbindelse avledet fra blod. Vanligvis hentes det fra ubedøvede fostre av storfe, og brukes som et supplement til basale vekstmedier. Det er en udefinert blanding med animalsk opprinnelse, noe som ... -
Exploring the Metabolic Adaptions to Hypoxia and the Role of mTOR and Hsp90 Inhibition in Hypoxia and Normoxia in Multiple Myeloma
Dahl, Anna Bergan (Master thesis, 2020)Hypoksi defineres som en tilstand der oksygennivået er redusert sammenlignet med det fysiologiske nivået. Ondartede kreftsvulster har ofte hypoksiske områder, fordi deres ukontrollerte proliferering og mangelfulle ... -
Fluopsin C for treating multidrug-resistant infections: In vitro activity aganist clinically important strains and in vivo efficacy against carbapenemase-producing Klebsiella pneumoniae
Navarro, Miguel Octavio Perez; Simionato, Ane Stefano; Perez, Juan Carlos Bedoya; Barazetti, Andre Riedi; Emiliano, Janaina; Niekawa, Erika Tyemi Goya; Andreata, Matheus Felipe de Lima; Modolon, Fluvio; Dealis, Mickely Liuti; Araujo, Eduardo Jose de Almeida; Carlos, Thalita Massi; Scarpelim, Odair Jose; Silva, Denise Brentan da; Chryssafidis, Andreas Lazaros; Bruheim, Per; Andrade, Galdino (Journal article; Peer reviewed, 2019)The increasing emergence of multidrug-resistant (MDR) organisms in hospital infectionsis causing a global public health crisis. The development of drugs with effective antibioticaction against such agents is of the highest ... -
Fluxome study of Pseudomonas fluorescens reveals major reorganisation of carbon flux through central metabolic pathways in response to inactivation of the anti-sigma factor MucA
Lien, Stina Katrine; Niedenführ, Sebastian; Sletta, Håvard; Nöh, Katharina; Bruheim, Per (Journal article; Peer reviewed, 2015)Background: The bacterium Pseudomonas fluorescens switches to an alginate-producing phenotype when the pleiotropic anti-sigma factor MucA is inactivated. The inactivation is accompanied by an increased biomass yield on ... -
Formation of Persister Cells and Metabolic Response to Sub-MIC Concentrations of Antibiotics in Escherichia coli
Falkenhaug, Hilde Nordby (Master thesis, 2021)Antibiotikaresistente bakterier har blitt et økende problem i behandling av flere ulike sykdommer, men mange antibiotikabehandlinger mislykkes også selv ved fravær av resistente bakterier. Bakterier kan overleve høye ... -
Functional validation of Frizzled-7 as a potential molecular target in glioblastoma multiforme
Solli, Elise (Master thesis, 2019)Glioblastoma multiforme (GBM) er en svært alvorlig type kreft, men en median overlevelsestid på kun 14 måndeder etter at diagnose er satt, selv om standard behandling igangsettes. Derfor er det et stort behov for nye ... -
G2 checkpoint siRNA screen in irradiated cancer cells: validation of candidate positive hits
Daviknes, Ingrid Marie Eriksen (Master thesis, 2011)Prior to this project, a high throughput assay was developed in order to perform automated RNAi screens with siRNA- libraries targeting potential regulators of the G2 checkpoint. The libraries were covering the human ... -
Gene expression studies and targeted metabolomics reveal disturbed serine, methionine, and tyrosine metabolism in early hypertensive nephrosclerosis
Øvrehus, Marius Altern; Bruheim, Per; Ju, Wenjun; Zelnick, Leila R.; Langlo, Knut Asbjørn Rise; Sharma, Kumar; de Boer, Ian H.; Hallan, Stein (Journal article; Peer reviewed, 2019)Introduction Hypertensive nephrosclerosis is among the leading causes of end-stage renal disease, but its pathophysiology is poorly understood. We wanted to explore early metabolic changes using gene expression and targeted ... -
Genome-scale models and consequences of mass constraints and availability of enzymes
Kumelj, Tjasa (Doctoral theses at NTNU;2021:294, Doctoral thesis, 2021)Genome-scale metabolic reconstructions offer a system-level view of metabolic networks with genetic basis and suggest mechanistic determination of cell's physiological homeostatic states. Consequently, they offer the ability ... -
Genotypic and Phenotypic Characterization of Norwegian Farmhouse Ale Yeast Cultures: A Domestication-Driven Evolution
Lægreid, Iselin Renée (Master thesis, 2017)There is an immense gap in our knowledge regarding biodiversity of yeasts and the available untapped yeasts that can be found in nature. Studying both domesticated and wild species is important as it can enable us to better ... -
Growth characteristics and metabolic changes in recombinant Escherichia coli with different plasmid copy numbers and expression system strengths
Mathiassen, Lise Femanger (Master thesis, 2022)Rekombinant protein produksjon har potensiale til å kunne produsere mange biofarmasøytiske produkter og industrielle enzymer av stor sosial og økonomisk betydning. Escherichia coli er en mye bruk vertsorganisme for rekombinant ... -
Growth Characterization and Mass Spectrometric Metabolic Profiling of Escherichia coli During Recombinant Protein Production
Mæhlum, Astfrid Helene Morka (Master thesis, 2021)Sammendrag Escherichia coli er en mye brukt vertsorganisme for rekombinant proteinproduksjon (RPP). Likevel presenterer denne bakterien fremdeles ulemper som hemmer proteinutbyttet. Utvikling og optimalisering av rekombinante ... -
Halogenated Pyrrolopyrimidines with Low MIC on Staphylococcus aureus and Synergistic Effects with an Antimicrobial Peptide
Olsen, Cecilie Elisabeth; Blindheim, Fredrik Heen; Søgaard, Caroline Krogh; Røst, Lise Marie; Singleton, Amanda; Bergum, Olaug Elisabeth Torheim; Bruheim, Per; Otterlei, Marit; Sundby, Eirik; Hoff, Bård Helge (Peer reviewed; Journal article, 2022)Currently, there is a world-wide rise in antibiotic resistance causing burdens to individuals and public healthcare systems. At the same time drug development is lagging behind. Therefore, finding new ways of treating ... -
Identification of Potential Cellular Pathways Involved in Mediating the Rapid Bactericidal Effects of the Novel β-clamp-targeting Antimicrobial Peptide Betatide
Wang, Emmy (Master thesis, 2023)Økende antimikrobiell resistens, samt redusert utvikling av nye antibiotika, har ført til en global utfordring som innebærer en stor økonomisk og sosial belastning. Betatide er et nytt antimikrobielt peptid som binder til ... -
Impact of CLN3 deficiency and Autophagy Lysosomal Pathway (ALP) in Neurodegeneration
Andersen, Vilde Nagelsaker (Master thesis, 2021)CLN3 Batten sykdom er en sjelden nevrodegenerativ lidelse som rammer barn. Sykdommen er forårsaket av mutasjoner i CLN3-genet, noe som blant annet fører til gradvis svekket syn som ender med blindhet, avtagende motoriske ... -
The impact of serum-free culture on HEK293 cells: From the establishment of suspension and adherent serum-free adaptation cultures to the investigation of growth and metabolic profiles
Jang, Mi; Pete, Ellen Sofie; Bruheim, Per (Peer reviewed; Journal article, 2022)Serum-free cultures are preferred for application in clinical cell therapy and facilitate the purification processes of bioproducts, such as vaccines and recombinant proteins. It can replace traditional cell culture - ... -
Impaired NDRG1 functions in Schwann cells cause demyelinating neuropathy in a dog model of Charcot-Marie-Tooth type 4D
Skedsmo, Fredrik; Espenes, Arild; Tranulis, Michael A.; Matiasek, Kaspar; Gunnes, Gjermund; Bjerkås, Inge; Moe, Lars; Røed, Susan Skogtvedt; Berendt, Mette; Fredholm, Merete; Rohdin, Cecilia; Shelton, G. Diane; Bruheim, Per; Stafsnes, Marit Hallvardsdotter; Bartosova, Zdenka; Hermansen, Lene Cecilie; Stigen, Øyvind; Jäderlund, Karin Hultin (Peer reviewed; Journal article, 2021)Mutations in the N-myc downstream-regulated gene 1 (NDRG1) cause degenerative polyneuropathy in ways that are poorly understood. We have investigated Alaskan Malamute dogs with neuropathy caused by a missense mutation in ... -
Improved phosphometabolome profiling applying isotope dilution strategy and capillary ion chromatography-tandem mass spectrometry
Stafsnes, Marit Hallvardsdotter; Røst, Lisa Marie; Bruheim, Per (Journal article; Peer reviewed, 2018)The phosphometabolome is comprised of all phosphorylated metabolites including the major metabolite classes sugar phosphates and nucleoside phosphates. Phosphometabolites are invaluable in any cell as a part of primary- ... -
Improvement of acetaldehyde production in Zymomonas mobilis by engineering of its aerobic metabolism
Kalnenieks, Uldis; Balodite, Elina; Strahler, Steffi; Strazdina, Inese; Rex, Julia; Pentjuss, Agris; Fuchino, Katsuya; Bruheim, Per; Rutkis, Reinis; Pappas, Katherine M.; Poole, Robert K.; Sawodny, Oliver; Bettenbrock, Katja (Journal article; Peer reviewed, 2019)Acetaldehyde is a valuable product of microbial biosynthesis, which can be used by the chemical industry as the entry point for production of various commodity chemicals. In ethanologenic microorganisms, like yeast or the ...