Browsing NTNU Open by Author "Flo, Trude Helen"
Now showing items 21-40 of 42
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Molecular tools and model systems for studying inflammasome activation
Kim, Haelin (Master thesis, 2019)Upon cellular stress or invasion by pathogens such as Mycobacterium tuberculosis (Mtb), inflammasomes are activated as an innate immune response, an important host-defense mechanism to resist pathogens. Activation of NLRP3 ... -
Mycobacterium smegmatis Vaccine Vector Elicits CD4+ Th17 and CD8+ Tc17 T Cells With Therapeutic Potential to Infections With Mycobacterium avium
Kannan, Nisha; Haug, Markus; Steigedal, Magnus; Flo, Trude Helen (Peer reviewed; Journal article, 2020)Mycobacterium avium (Mav) complex is increasingly reported to cause non-tuberculous infections in individuals with a compromised immune system. Treatment is complicated and no vaccines are available. Previous studies have ... -
N-3 PUFAs induce inflammatory tolerance by formation of KEAP1-containing SQSTM1/p62-bodies and activation of NFE2L2
Mildenberger, Jennifer; Johansson, Ida; Sergin, Ismail; Kjøbli, Eli; Damås, Jan Kristian; Razani, Babak; Flo, Trude Helen; Bjørkøy, Geir (Journal article; Peer reviewed, 2017)Inflammation is crucial in the defense against infections but must be tightly controlled to limit detrimental hyperactivation. Our diet influences inflammatory processes and omega-3 polyunsaturated fatty acids (n-3 PUFAs) ... -
Persistent mycobacteria evade an antibacterial program mediated by phagolysosomal TLR7/8/MyD88 in human primary macrophages
Gidon, Alexandre; Åsberg, Signe; Louet, Claire; Ryan, Liv; Haug, Markus; Flo, Trude Helen (Journal article; Peer reviewed, 2017)Pathogenic mycobacteria reside in macrophages where they avoid lysosomal targeting and degradation through poorly understood mechanisms proposed to involve arrest of phagosomal maturation at an early endosomal stage. A ... -
Photochemical internalization of peptide antigens provides a novel strategy to realize therapeutic cancer vaccination
Haug, Markus; Brede, Gaute; Håkerud, Monika; Nedberg, Anne Grete; Gederaas, Odrun Arna; Flo, Trude Helen; Edwards, Victoria Tudor; Selbo, Pål Kristian; Høgset, Anders; Halaas, Øyvind (Journal article; Peer reviewed, 2018)Effective priming and activation of tumor-specific CD8+ cytotoxic T lymphocytes (CTLs) is crucial for realizing the potential of therapeutic cancer vaccination. This requires cytosolic antigens that feed into the MHC class ... -
Plasma membrane damage causes NLRP3 activation and pyroptosis during Mycobacterium tuberculosis infection
Beckwith, Kai Sandvold; Beckwith, Marianne Sandvold; Ullmann, Sindre; Sætra, Ragnhild Sofie Ragnhildstveit; Kim, Haelin; Marstad, Anne; Åsberg, Signe Elisabeth; Strand, Trine Aakvik; Haug, Markus; Niederweis, Michael; Stenmark, Harald Alfred; Flo, Trude Helen (Peer reviewed; Journal article, 2020)Mycobacterium tuberculosis is a global health problem in part as a result of extensive cytotoxicity caused by the infection. Here, we show how M. tuberculosis causes caspase-1/NLRP3/gasdermin D-mediated pyroptosis of human ... -
The Proteomic Landscape of Resting and Activated CD4+ T Cells Reveal Insights into Cell Differentiation and Function
Subbannayya, Yashwanth; Haug, Markus; Pinto, Sneha; Mohanty, Varshasnata; Meås, Hany Zakaria; Flo, Trude Helen; Prasad, T. S. Keshava; Kandasamy, Richard Kumaran (Peer reviewed; Journal article, 2020)CD4+ T cells (T helper cells) are cytokine-producing adaptive immune cells that activate or regulate the responses of various immune cells. The activation and functional status of CD4+ T cells is important for adequate ... -
Pyruvate Supports RET-Dependent Mitochondrial ROS Production to Control Mycobacterium avium Infection in Human Primary Macrophages
Røst, Lisa M.; Louet, Claire; Bruheim, Per; Flo, Trude Helen; Gidon, Alexandre (Peer reviewed; Journal article, 2022)Macrophages deploy a variety of antimicrobial programs to contain mycobacterial infection. Upon activation, they undergo extensive metabolic reprogramming to meet an increase in energy demand, but also to support immune ... -
Receptors involved in cell activation by defined uronic acid polymers and bacterial components
Flo, Trude Helen (Dissertations at the Faculty of Medicine, 0805-7680; 187, Doctoral thesis, 2001)PAPER 1 In the first paper we show that reducing the average molecular weight from ~350 kDa to <6kDa by acid hydrolysis diminished the cell-stimulating activity of poly-M, measured as TNFproduction from human monocytes. ... -
Regulation of autophagy during Mycobacterium avium infection in primary human macrophages
Shrestha, Birendra Kumar (Master thesis, 2013)The main aim of this project was to study the induction and regulation of autophagy during Mycobacterium avium infection in primary human macrophages. Specifically, we wanted to elucidate the role of Toll-like receptors ... -
Seeing a Mycobacterium-Infected Cell in Nanoscale 3D: Correlative Imaging by Light Microscopy and FIB/SEM Tomography
Beckwith, Marianne; Beckwith, Kai Sandvold; Sikorski, Pawel; Skogaker, Nan Elisabeth Tostrup; Flo, Trude Helen; Halaas, Øyvind (Journal article; Peer reviewed, 2015)Mycobacteria pose a threat to the world health today, with pathogenic and opportunistic bacteria causing tuberculosis and non-tuberculous disease in large parts of the population. Much is still unknown about the interplay ... -
Sensing of HIV-1 by TLR8 activates human T cells and reverses latency
Meås, Hany Zakaria; Haug, Markus; Beckwith, Marianne Sandvold; Louet, Claire; Ryan, Liv; Hu, Zhenyi; Landskron, Johannes; Nordbø, Svein Arne; Tasken, Kjetil; Yin, Hang; Damås, Jan Kristian; Flo, Trude Helen (Journal article; Peer reviewed, 2020)During HIV infection, cell-to-cell transmission results in endosomal uptake of the virus by target CD4+ T cells and potential exposure of the viral ssRNA genome to endosomal Toll-like receptors (TLRs). TLRs are instrumental ... -
Studying the effect of Keap1 on innate immune signaling pathways in Mycobacterium avium infection
Chau, Do Ngoc Phuc (Master thesis, 2013)The Mycobacterium species include pathogens causing serious diseases in mammals. Mycobacterium avium complex (MAC), while it has low virulence to normal people, it is a high potential pathogen causing TB-like disease in ... -
Targeted correlative light and electron microscopy in the study of host-pathogen interactions
Beckwith, Marianne S (Doktoravhandlinger ved NTNU;2018:89, Doctoral thesis, 2018)Abstract Tuberculosis (TB) and HIV are the two deadliest infectious diseases in the world today. 1,8 million people died from TB in 2015, 400.000 of which were HIV positive. Drug resistant TB strains are rapidly spreading, ... -
The Drug, the Bug and the Macrophage: Interactions between antibiotics, mycobacteria and macrophages at the single cell and subcellular level
Åsberg, Signe Elisabeth (Doctoral theses at NTNU;2018:329, Doctoral thesis, 2018) -
The Interplay of Oxidative Stress Responses and Autophagy in Inflammation
Mildenberger, Jennifer Melanie (Doctoral theses at NTNU;2017:186, Doctoral thesis, 2017)To assure homeostasis, cells have to continuously adapt to exogenous and endogenous threats. This is achieved by response mechanisms like the oxidative stress response, autophagy and inflammation. The oxidative stress ... -
The Role of KEAP1 in Regulating Inflammation and Antigen-Presentation in Response to Mycobacterium avium Infection in Macrophages
Bjørnvall, Christina Dybdrodt (Master thesis, 2014) -
The role of the innate immune sensor STING in T cells
Rønningstad, Åshild (Master thesis, 2023)Signalveien cyclic GMP-AMP-synthase (cGAS)-stimulator of interferons (STING) er en viktig del av det medfødte immunforsvaret. Mønstergjenkjenningsreseptorene (PPR) uttrykkes i mange celletyper og kan oppdage DNA- og ... -
TLR8 senses Staphylococcus aureus RNA in human primary monocytes and macrophages and induces IFN-β production via a TAK1-IKKβ-IRF5 signaling pathway
Bergstrøm, Bjarte; Aune, Marie Hjelmseth; Awuh, Jane Atesoh; Kojen, June Frengen; Blix, Kjetil Jordahl; Ryan, Liv; Flo, Trude Helen; Mollnes, Tom Eirik; Espevik, Terje; Stenvik, Jørgen (Journal article, 2015) -
The Tumor Necrosis Factor Alpha and Interleukin 6 Auto-paracrine Signaling Loop Controls Mycobacterium avium Infection via Induction of IRF1/IRG1 in Human Primary Macrophages
Gidon, Alexandre; Louet, Claire; Røst, Lisa M.; Bruheim, Per; Flo, Trude Helen (Peer reviewed; Journal article, 2021)Macrophages sense and respond to pathogens by induction of antimicrobial and inflammatory programs to alert other immune cells and eliminate the infectious threat. We have previously identified the transcription factor ...