Browsing NTNU Open by Author "Brautaset, Trygve"
Now showing items 1-20 of 66
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6-Phosphofructokinase and ribulose-5-phosphate 3-epimerase in methylotrophic Bacillus methanolicus ribulose monophosphate cycle
Le, Simone Balzer; Heggset, Tonje Marita Bjerkan; Haugen, Tone; Nærdal, Ingemar; Brautaset, Trygve (Journal article; Peer reviewed, 2017)d-Ribulose-5-phosphate-3-epimerase (RPE) and 6-phosphofructokinase (PFK) catalyse two reactions in the ribulose monophosphate (RuMP) cycle in Bacillus methanolicus. The B. methanolicus wild-type strain MGA3 possesses two ... -
A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli
Balzer, Simone; Kucharova, Veronika; Megerle, Judith; Lale, Rahmi; Brautaset, Trygve; Valla, Svein (Journal article; Peer reviewed, 2013)Abstract Background Production of recombinant proteins in bacteria for academic and commercial purposes is a well established field; however the outcomes of process developments for specific proteins are still often ... -
A novel expression system for lytic polysaccharide monooxygenases
Courtade, Gaston; Le, Simone Balzer; Sætrom, Gerd Inger; Brautaset, Trygve; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2017)Lytic polysaccharide monooxygenases (LPMOs) are key enzymatic players of lignocellulosic biomass degradation processes. As such, they have been introduced in cellulolytic cocktails for more efficient and less expensive ... -
Advancement of the XylS/Pm Expression System in Gram-positive Bacteria Evaluated in Bacillus subtilis
Burwash,Katrina (Master thesis, 2019)Escherichia coli (E. coli) is one of the best characterized prokaryotes and therefore one of the most popular hosts used for heterologous protein expression. Today, the XylS/Pm expression system is predominantly used in ... -
Advancements in Construction of XylS/Pm Expression Vectors in Gram-positive Bacteria - Evaluated in Bacillus subtilis and Lactobacillus plantarum
Istre, Mette Stausland (Master thesis, 2018)Escherichia coli (E. coli) is the most commonly used host for expression of recombinant proteins. A large fraction of recombinant proteins produced in E. coli are stored in an insoluble form, making ... -
An NMR investigation of lytic polysaccharide monooxygenases
Courtade, Gaston (Doctoral theses;2018:337, Doctoral thesis, 2018)Bioeconomy requires sustainable sources of biomass (e.g. chitin and cellulose) to use as energy, feed and biomaterials. In the quest of biomass conversion, lytic polysaccharide monooxygenases (LPMOs) are key enzymatic ... -
Bacillus methanolicus MGA3 as host for methanol-based production of L-tryptophan-derived specialty compounds violacein, indole and tryptamine
Korneliussen, Daniel (Master thesis, 2022)Interesse i bruk av en-karbon forbindelser, som metanol, som energikilde for produksjon av bioteknologiske relevante stoffer har økt de siste årene. Metanol er en lett tilgjengelig forbindelse som assimileres av en gruppe ... -
Centre for Digital Life Norway - Annual report 2017
Johansen, Finn-Eirik; Brautaset, Trygve; Aune, Marie Hjelmseth; Patriksson, Alexandra; Falkenberg, Liv Eggset; Kleppe, Rune; Dick, Gunnar; Strand, Berit Løkensgard; Eijsink, Vincent; Røyne, Anja; Goksøyr, Anders; Svendsen, John Sigurd Mjøen; Fyhn, Marianne; Vik, Jon Olav; Wentzel, Alexander; Aksnes, Astrid; Bruheim, Per; Carlsen, Sven Magnus; Åm, Heidrun (Research report, 2018) -
Composition analysis and minimal treatments to solubilize 6 polysaccharides from the brown seaweed Laminaria digitata 7 for microbial growth of thermophiles
Allahgoli, Leila; Hakvåg, Sigrid; Aasen, Inga Marie; Brautaset, Trygve; Eva, Nordberg Karlsson (Peer reviewed; Journal article, 2020)Brown macroalgae (Phaeophyta) hold high potential as feedstock for biorefineries due to high biomass productivity and carbohydrate content. They are, however, a challenging, unconventional feedstock for microbial refining ... -
Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production
Gawin, Agnieszka; Peebo, Karl; Sebastian, Hans; Ertesvåg, Helga; Irla, Marta Katarzyna; Neubauer, Peter; Brautaset, Trygve (Journal article; Peer reviewed, 2019)Background Bacteria are widely used as hosts for recombinant protein production due to their rapid growth, simple media requirement and ability to produce high yields of correctly folded proteins. Overproduction of ... -
Construction of XylS/Pm Expression Vectors for Bacillus subtilis - Evaluated in Bacillus subtilis and Lactobacillus plantarum
Hverven, Sara Kristine Kildal (Master thesis, 2017)Today, Escherichia coli (E.coli) is widely used as recombinant protein expression host. However, other species could be more suitable to express some recombinant proteins. In the present study, plasmids with XylS/Pm ... -
Corrigendum: Genome-Based Genetic Tool Development for Bacillus methanolicus: Theta- and Rolling Circle-Replicating Plasmids for Inducible Gene Expression and Application to Methanol-Based Cadaverine Production
Irla, Marta Katarzyna; Heggeset, Tonje Marita Bjerkan; Nærdal, Ingemar; Paul, Lidia; Haugen, Tone; Le, Simone Balzer; Brautaset, Trygve; Wendisch, Volker F. (Journal article; Peer reviewed, 2019) -
Developing a riboswitch-mediated regulatory system for metabolic flux control in thermophilic bacillus methanolicus
Irla, Marta Katarzyna; Hakvåg, Sigrid; Brautaset, Trygve (Journal article; Peer reviewed, 2021) -
Development of a markerless tool for targeted deletion of chromosomal genes in the thermotolerant and methylotrophic bacterium Bacillus methanolicus
Langlo, Jesper (Master thesis, 2020)Bakterien Bacillus methanolicus (B. methanolicus) utgjør en svært interessant kandidat innen industriell bioteknologi, i hovedsak grunnet dens termofile og metylotrofiske egenskaper, og for at den kan produsere høy-verdi ... -
Draft genome sequence of the docosahexaenoic acid producing thraustochytrid Aurantiochytrium sp. T66
Liu, Bin; Ertesvåg, Helga; Aasen, Inga Marie; Vadstein, Olav; Brautaset, Trygve; Heggeset, Tonje Marita Bjerkan (Journal article; Peer reviewed, 2016)Thraustochytrids are unicellular, marine protists, and there is a growing industrial interest in these organisms, particularly because some species, including strains belonging to the genus Aurantiochytrium, accumulate ... -
Effects of Sec-Pathway Modifications on Periplasmatic Translocation of Recombinant Proteins in Escherichia coli
Elvheim, Andrea Iselin (Master thesis, 2018)Recombinant proteins are expressed for use in both research, industry and medicine. The Gram-negative bacteria Escherichia coli (E. coli) is often used as an expression host. It has several properties making it a suitable ... -
Effects of Targeted Knockout Mutations on Translational Capacities in Escherichia coli - Establishing Methods to Increase and Evaluate Translational Capacities in E. coli BW25113
Jønland, Mats Håkon Grøn (Master thesis, 2017)The overall aim of this thesis was to investigate how genome engineering might be used to generate Escherichia coli strains with increased capacities for recombinant protein production. As translation constitute a possible ... -
Engineering the genome of Escherichia coli BL21 for improved secretory production of recombinant proteins
Hansen, Sine Alise Hartvigsen (Master thesis, 2019)Et økt fokus på rekombinante legemidler er sett i den farmasøytiske industrien. De fleste rekombinante legemidler stammer fra eukaryoter, og krever posttranslasjons-modifikasjoner slik som dannelse av disulfid-bindinger. ... -
Establishing 3-hydroxypropionic acid production in Bacillus methanolicus MGA3 by heterologous expression of malonyl coenzyme A reductase and malonic semialdehyde reductase
Jacobsen, Robert Torsvik (Master thesis, 2022)3-Hydroxypropionsyre regnes å være en av de mest sentrale biobaserte kjemikaliene som kan produseres fra fornybare karbohydrater. Syren brukes som en forløper for flere ulike kjemiske forbindelser i produksjon av forbruksvarer. ... -
Establishing new XylS/Pm based Gram-Negative Protein Production Strains - Evaluating Pseudoalteromonas haloplanktis and Pseudomonas putida
Strypet, Goro Malene Brennmoen (Master thesis, 2018)To date, Escherichia coli is the most used bacterial host for recombinant protein production. Some proteins are, however, hard to express in this species due to a reducing intracellular environment and inclusion body ...