dc.contributor.author | Magalhães, Rui | |
dc.contributor.author | Guardiola, Francisco Abellán | |
dc.contributor.author | Guerreiro, Inês | |
dc.contributor.author | Fontinha, Filipa | |
dc.contributor.author | Moutinho, Sara | |
dc.contributor.author | Serra, Cláudia Reis | |
dc.contributor.author | Olsen, Rolf Erik | |
dc.contributor.author | Peres, Helena | |
dc.contributor.author | Oliva-Teles, Aires | |
dc.date.accessioned | 2024-01-19T08:32:38Z | |
dc.date.available | 2024-01-19T08:32:38Z | |
dc.date.created | 2022-11-08T09:44:39Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Aquaculture Research. 2022, 53 (17), 6007-6019. | en_US |
dc.identifier.issn | 1355-557X | |
dc.identifier.uri | https://hdl.handle.net/11250/3112667 | |
dc.description.abstract | The immunomodulatory effect of different dietary arachidonic acid (ARA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) ratios was evaluated in gilthead sea bream juveniles after intraperitoneal injection (i.p.) with formalin-killed Photobacterium damselae subsp. piscicida (Phdp). Fish were fed with four isoproteic (47% crude protein) and isolipidic (18% crude lipids) diets with the following long-chain polyunsaturated fatty acids (LC-PUFA) ratios: Diet A (2.0% ARA: 0.2% EPA: 0.1% DHA); Diet B (1.0% ARA: 0.4% EPA: 0.4% DHA); Diet C (0% ARA: 0.6% EPA: 0.6% DHA); Diet D (0% ARA: 0.3% EPA: 1.5% DHA). After being fed for 56 days with the experimental diets, fish from each dietary treatment were randomly selected and i.p. injected with 0.2 ml of killed Phdp (1 × 108 CFU ml−1) or phosphate-buffered saline (control group). Four and twenty-four hours (i.e., twice) after the i.p., the haematological profile, plasma humoral innate immune parameters, and the intestine expression of immune-related genes were assessed. Results showed that fish fed diet D (high DHA content) had increased complement activity and higher intestinal expression of cyclooxygenase 2 (cox2) and interleukin 1β (il-1β) at 4 and 24 hours after i.p., as well as increased intestinal tumour necrosis factor α (tnfα) expression at 4 hours. On the other hand, fish fed with diet A (high ARA content) showed higher plasma bactericidal activity against Phdp than fish fed with diet D. Overall, the results of this study suggest a positive modulatory effect of DHA in response to the pathogenic agent. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Wiley | en_US |
dc.title | Immunomodulatory effect of different dietary ARA/EPA/DHA ratios in gilthead sea bream (Sparus aurata) juveniles after infection with Photobacterium damselae subsp. piscicida | en_US |
dc.title.alternative | Immunomodulatory effect of different dietary ARA/EPA/DHA ratios in gilthead sea bream (Sparus aurata) juveniles after infection with Photobacterium damselae subsp. piscicida | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright © 2022 John Wiley & Sons, Inc | en_US |
dc.source.pagenumber | 6007-6019 | en_US |
dc.source.volume | 53 | en_US |
dc.source.journal | Aquaculture Research | en_US |
dc.source.issue | 17 | en_US |
dc.identifier.doi | 10.1111/are.16070 | |
dc.identifier.cristin | 2070375 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |