dc.contributor.author | Lehnert, Sarah | |
dc.contributor.author | Sikorski, Pawel | |
dc.date.accessioned | 2022-04-01T12:19:53Z | |
dc.date.available | 2022-04-01T12:19:53Z | |
dc.date.created | 2021-03-04T09:37:28Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Materials Science and Engineering C: Materials for Biological Applications. 2021, 121 . | en_US |
dc.identifier.issn | 0928-4931 | |
dc.identifier.uri | https://hdl.handle.net/11250/2989307 | |
dc.description.abstract | The application of microspheres instead of bulk hydrogels in cell-laden biomaterials offers multiple advantages such as a high surface-to-volume-ratio and, consequently, a better nutrition and oxygen transfer to and from cells. The preparation of inert alginate microspheres is facile, quick, and well-established and the fabrication of alginate-collagen microspheres has been previously reported. However, no detailed characterization of the collagen fibrillogenesis in the alginate matrix is available. We use second-harmonic imaging microscopy reflection confocal microscopy and turbidity assay to study the assembly of collagen in alginate microspheres. We show that the assembly of collagen fibers in a gelled alginate matrix is a complex process that can be aided by addition of small polar molecules, such as glycine and by a careful selection of the gelling buffer used to prepare alginate hydrogels. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Tailoring the assembly of collagen fibers in alginate microspheres | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.pagenumber | 11 | en_US |
dc.source.volume | 121 | en_US |
dc.source.journal | Materials Science and Engineering C: Materials for Biological Applications | en_US |
dc.identifier.doi | 10.1016/j.msec.2020.111840 | |
dc.identifier.cristin | 1895522 | |
dc.relation.project | Norges forskningsråd: 262893 | en_US |
dc.relation.project | Norges forskningsråd: 295864 | en_US |
cristin.ispublished | true | |
cristin.fulltext | postprint | |
cristin.qualitycode | 1 | |