"Insights into codon usage bias and development of synthetic biology tools for advancing gene and pathway expression in microorganisms"

Abstract

The thesis explores the feasibility of recoding genomes in algae to expand the genetic code to non-canonical amino acids. The thesis investigates the features of the chloroplast’s genetic code to understand how it can be modified. However, genome recoding strongly affects gene expression, which perturbs the metabolism of the cell. The thesis provides several genetic tools that circumvent gene expression issues and equilibrate cell fitness. These tools are powerful assets for synthetic biology and biotechnology research aiming to produce valuable molecules."