Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli
| dc.contributor.author | Helgesen, Emily | |
| dc.contributor.author | Sætre, Frank Ove | |
| dc.contributor.author | Skarstad, Kirsten Jane | |
| dc.date.accessioned | 2021-09-10T08:35:32Z | |
| dc.date.available | 2021-09-10T08:35:32Z | |
| dc.date.created | 2021-04-07T13:33:34Z | |
| dc.date.issued | 2021 | |
| dc.identifier.citation | Scientific Reports. 2021, 11 (1), 1-8. | en_US |
| dc.identifier.issn | 2045-2322 | |
| dc.identifier.uri | https://hdl.handle.net/11250/2775146 | |
| dc.description.abstract | Topoisomerase IV (TopoIV) is a vital bacterial enzyme which disentangles newly replicated DNA and enables segregation of daughter chromosomes. In bacteria, DNA replication and segregation are concurrent processes. This means that TopoIV must continually remove inter-DNA linkages during replication. There exists a short time lag of about 10–20 min between replication and segregation in which the daughter chromosomes are intertwined. Exactly where TopoIV binds during the cell cycle has been the subject of much debate. We show here that TopoIV localizes to the origin proximal side of the fork trailing protein SeqA and follows the movement pattern of the replication machinery in the cell. | en_US |
| dc.language.iso | eng | en_US |
| dc.publisher | Springer Nature | en_US |
| dc.rights | Navngivelse 4.0 Internasjonal | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
| dc.title | Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli | en_US |
| dc.type | Peer reviewed | en_US |
| dc.type | Journal article | en_US |
| dc.description.version | publishedVersion | en_US |
| dc.source.pagenumber | 1-8 | en_US |
| dc.source.volume | 11 | en_US |
| dc.source.journal | Scientific Reports | en_US |
| dc.source.issue | 1 | en_US |
| dc.identifier.doi | 10.1038/s41598-020-80043-4 | |
| dc.identifier.cristin | 1902730 | |
| dc.relation.project | Helse Sør-Øst RHF: 2012062 | en_US |
| dc.description.localcode | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. | en_US |
| dc.source.articlenumber | 474 | en_US |
| cristin.ispublished | true | |
| cristin.fulltext | original | |
| cristin.qualitycode | 1 |
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