dc.contributor.author | Tang, Wannan | |
dc.contributor.author | Zillmann, Uwe | |
dc.contributor.author | Sprengel, Rolf | |
dc.date.accessioned | 2021-02-09T09:43:54Z | |
dc.date.available | 2021-02-09T09:43:54Z | |
dc.date.created | 2021-01-22T22:05:38Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Frontiers in Behavioral Neuroscience. 2020, 14:115 1-10. | en_US |
dc.identifier.issn | 1662-5153 | |
dc.identifier.uri | https://hdl.handle.net/11250/2726811 | |
dc.description.abstract | Viral-transduced gene expression is the current standard for cell-type-specific labeling and cell tacking in experimental neuroscience. To achieve widespread gene expression, a viral delivery method to neonatal rodents was introduced more than two decades ago. Most of those neonatal viral vector injection-based gene transduction methods in mice used deep hypothermia for anesthesia, which was reported to be associated with behavioral impairments. To explore other options for neonatal viral applications, we applied a combination of Medetomidine, Midazolam, and Fentanyl (MMF), each of which can be antagonized by a specific antagonist. Later in their adulthood, we found that adult mice, that received the MMF-induced anesthesia, combined with virus-injected into the brain at postnatal day 2, showed similar performance in all behavioral tasks tested, including tasks for motor coordination, anxiety-related tasks, and spatial memory when compared to adult naïve littermates. This demonstrates that MMF anesthesia could be safely applied to mice for neonatal viral transduction at P2. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Frontiers Media | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Alternative anesthesia of neonatal mice for global rAAV delivery in the brain with non-detectable behavioral interference in adults | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.pagenumber | 1-10 | en_US |
dc.source.volume | 14:115 | en_US |
dc.source.journal | Frontiers in Behavioral Neuroscience | en_US |
dc.identifier.doi | 10.3389/fnbeh.2020.00115 | |
dc.identifier.cristin | 1877517 | |
dc.relation.project | Norges forskningsråd: 262552 | en_US |
dc.description.localcode | Copyright © 2020 Tang, Zillmann and Sprengel. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |