dc.contributor.author | Bjorgan, Asgeir | |
dc.contributor.author | Randeberg, Lise Lyngsnes | |
dc.date.accessioned | 2019-05-23T12:29:04Z | |
dc.date.available | 2019-05-23T12:29:04Z | |
dc.date.created | 2019-03-06T13:50:02Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Proceedings of SPIE. 2019, 10873 | nb_NO |
dc.identifier.issn | 0277-786X | |
dc.identifier.uri | http://hdl.handle.net/11250/2598589 | |
dc.description.abstract | The spectral and spatial resolution of hyperspectral imaging is useful for investigation of tissue autofluorescence. The low-light, noisy conditions in fluorescence imaging usually necessitates noise removal for extraction of precise spectral signatures and peak shifts. However, noise removal techniques like low-pass filtering or the Maximum Noise Fraction transform might discard information or distort spectral features. In this study, smoothing splines is proposed as an alternative technique to avoid spectral distortion in analysis of hyperspectral fluorescence images in the wavelength range 400-1000 nm. Continuous tuning parameters and use of natural cubic splines makes the method advantageous for unbiased peak extraction. The method was tested on ex vivo images of atherosclerosis lesions and simulations. The method was used to estimate autofluorescence peak shifts, and found to perform well in comparison with MNF. | nb_NO |
dc.language.iso | eng | nb_NO |
dc.publisher | Society of Photo Optical Instrumentation Engineers (SPIE) | nb_NO |
dc.title | Application of smoothing splines for spectroscopic analysis in hyperspectral images | nb_NO |
dc.type | Journal article | nb_NO |
dc.type | Peer reviewed | nb_NO |
dc.description.version | publishedVersion | nb_NO |
dc.source.volume | 10873 | nb_NO |
dc.source.journal | Proceedings of SPIE, the International Society for Optical Engineering | nb_NO |
dc.identifier.doi | https://doi.org/10.1117/12.2506618 | |
dc.identifier.cristin | 1682608 | |
dc.description.localcode | © 2019 Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. | nb_NO |
cristin.unitcode | 194,63,35,0 | |
cristin.unitname | Institutt for elektroniske systemer | |
cristin.ispublished | true | |
cristin.fulltext | preprint | |
cristin.qualitycode | 1 | |