Cell wall integrity modulates Arabidopsis thaliana cell cycle gene expression in a cytokinin- and nitrate reductase-dependent manner

Abstract

During plant growth and defense, cell cycle activity needs to be coordinated with cell wall integrity. Little is known about how coordination is achieved. Here we investigated coordination in Arabidopsis thaliana seedlings by studying the impact of cell wall damage (CWD, caused by cellulose biosynthesis inhibition) on cytokinin homeostasis, cell cycle gene expression and shape in root tips. CWD inhibited cell cycle gene expression and increased transition zone cell width in an osmo-sensitive manner. These results were correlated with CWD-induced, osmo-sensitive changes in cytokinin homeostasis. Expression of CYTOKININ OXIDASE/DEHYDROGENASE2 and 3 (CKX2, CKX3), encoding cytokinin-degrading enzymes was induced by CWD and reduced by osmoticum treatment. In nitrate reductase1 nitrate reductase2 (nia1 nia2) seedlings, neither CKX2 and CKX3 transcript levels were increased nor cell cycle gene expression repressed by CWD. Moreover, established CWD-induced responses like jasmonic acid, salicylic acid and lignin production, were also absent, implying a central role of NIA1- and NIA2-mediated processes in regulation of CWD responses. These results suggest that CWD enhances cytokinin degradation rates through a NIA1 and NIA2-mediated process, subsequently attenuating cell cycle gene expression.