Browsing NTNU Open by Author "Beckwith, Kai Sandvold"
Now showing items 1-12 of 12
-
Dissolution of copper mineral phases in biological fluids and the controlled release of copper ions from mineralized alginate hydrogels
Bassett, David; Madzovska, Ivana; Beckwith, Kai Sandvold; Melø, Thor Bernt; Sikorski, Pawel; Obradovic, Bojana (Journal article; Peer reviewed, 2015)Here we investigate the dissolution behaviour of copper minerals contained within biocompatible alginate hydrogels. Copper has a number of biological effects and has most recently been evaluated as an alternative to expensive ... -
Effects of Nanopillar Arrays on Cell Motility - A Study of Cell-Nanotopography Interactions
Schanke, Rasmus (Master thesis, 2015)The interplay between cells and engineered high-aspect ratio nanos- tructures is emerging as an interesting and promising field within life sciences. Specific cell responses arise as a result of interactions with these ... -
Electron Beam Lithography Fabrication of SU-8 Polymer Structures for Cell Studies
Vinje, Jakob; Beckwith, Kai Sandvold; Sikorski, Pawel (Journal article; Peer reviewed, 2020)Flat surfaces decorated with micro- and nanostructures are important tools in biomedical research used to control cellular shape, in studies of mechanotransduction, membrane mechanics, cell migration and cellular interactions ... -
Human Toll-like Receptor 8 (TLR8) Is an Important Sensor of Pyogenic Bacteria, and Is Attenuated by Cell Surface TLR Signaling
Moen, Siv Helen; Ehrnström, Birgitta; Kojen, June Frengen; Yurchenko, Mariya; Beckwith, Kai Sandvold; Afset, Jan Egil; Damås, Jan Kristian; Zhenyi, Hu; Hang, Yin; Espevik, Terje; Stenvik, Jørgen (Journal article; Peer reviewed, 2019)TLR8 is an endosomal sensor of RNA degradation products in human phagocytes, and is involved in the recognition of viral and bacterial pathogens. We previously showed that in human primary monocytes and monocyte derived ... -
Influence of Nanopillar Arrays on Fibroblast Motility, Adhesion, and Migration Mechanisms
Beckwith, Kai Sandvold; Ullmann, Sindre; Vinje, Jakob; Sikorski, Pawel (Journal article; Peer reviewed, 2019)Surfaces decorated with high aspect ratio nanostructures are a promising tool to study cellular processes and design novel devices to control cellular behavior. However, little is known about the dynamics of cellular ... -
Membrane damage and inflammasome activation during Mtb infection
Sætra, Ragnhild Sofie Ragnhildstveit (Master thesis, 2019)Tuberkulose, som forårsakes av Mycobacterium tuberculosis (Mtb), er et globalt helseproblem, og økt forståelse av de molekylære mekanismene bak Mtb-infeksjon er viktig. På enkeltcellenivå kan Mtb-infeksjon føre til aktivering ... -
Micro- and nanostructured devices for cell studies
Beckwith, Kai Sandvold (Doctoral thesis at NTNU;2015:240, Doctoral thesis, 2015)Cell culture is a fundamental and valuable tool for modern biomedical research. The purpose of this work has been to expand the possibilities and methods of cell culture through engineering of the cell culture substrate. ... -
Molecular tools and model systems for studying inflammasome activation
Kim, Haelin (Master thesis, 2019)Upon cellular stress or invasion by pathogens such as Mycobacterium tuberculosis (Mtb), inflammasomes are activated as an innate immune response, an important host-defense mechanism to resist pathogens. Activation of NLRP3 ... -
Plasma membrane damage causes NLRP3 activation and pyroptosis during Mycobacterium tuberculosis infection
Beckwith, Kai Sandvold; Beckwith, Marianne Sandvold; Ullmann, Sindre; Sætra, Ragnhild Sofie Ragnhildstveit; Kim, Haelin; Marstad, Anne; Åsberg, Signe Elisabeth; Strand, Trine Aakvik; Haug, Markus; Niederweis, Michael; Stenmark, Harald Alfred; Flo, Trude Helen (Peer reviewed; Journal article, 2020)Mycobacterium tuberculosis is a global health problem in part as a result of extensive cytotoxicity caused by the infection. Here, we show how M. tuberculosis causes caspase-1/NLRP3/gasdermin D-mediated pyroptosis of human ... -
Seeing a Mycobacterium-Infected Cell in Nanoscale 3D: Correlative Imaging by Light Microscopy and FIB/SEM Tomography
Beckwith, Marianne; Beckwith, Kai Sandvold; Sikorski, Pawel; Skogaker, Nan Elisabeth Tostrup; Flo, Trude Helen; Halaas, Øyvind (Journal article; Peer reviewed, 2015)Mycobacteria pose a threat to the world health today, with pathogenic and opportunistic bacteria causing tuberculosis and non-tuberculous disease in large parts of the population. Much is still unknown about the interplay ... -
Toll-Like Receptor 8 Is a Major Sensor of Group B Streptococcus But Not Escherichia coli in Human Primary Monocytes and Macrophages
Ehrnstrøm, Birgitta; Beckwith, Kai Sandvold; Yurchenko, Mariya; Moen, Siv Helen; Frengen Kojen, June; Lentini, Germana; Teti, G.; Damås, Jan Kristian; Espevik, Terje; Stenvik, Jørgen (Journal article; Peer reviewed, 2017)TLR8 is the major endosomal sensor of degraded RNA in human monocytes and macrophages. It has been implicated in the sensing of viruses and more recently also bacteria. We previously identified a TLR8-IFN regulatory factor ... -
Tunable high aspect ratio polymer nanostructures for cell interfaces
Beckwith, Kai Sandvold; Cooil, Simon Phillip; Wells, Justin; Sikorski, Pawel (Journal article; Peer reviewed, 2015)Nanoscale topographies and chemical patterns can be used as synthetic cell interfaces with a range of applications including study and control of cellular processes. Herein, we describe the fabrication of high aspect ratio ...